牡丹胚珠的结构观察与珠心的非酶法分离

为了获得牡丹胚珠内的珠心，在对牡丹胚珠的结构进行观察的基础上，研究了牡丹珠心的非酶法分离技术。以新鲜和酒精溶液固定的牡丹胚珠为材料，利用荧光显微镜、84消毒液的整体透明技术及切片技术，研究了牡丹胚珠的结构，并以新鲜的牡丹胚珠为材料，在解剖镜下利用胶块对牡丹的珠心等结构进行了非酶法分离。结果表明：在牡丹胚珠的2层珠被中，外珠被很厚，容易除去，而内珠被的外层容易与珠被绒毡层脱离并除去。在牡丹的胚珠中，只有珠被绒毡层能够发出清晰的荧光。在解剖镜下，利用胶块依次将牡丹胚珠的外珠被、内珠被外层和珠被绒毡层除去后，可分离出具有原有形态的牡丹珠心。

Structural Observation of Ovule and Non-enzymatical Isolation of Nucellus in Paeonia suffruticosa

In order to obtain the nucellus of Paeonia suffruticosa, the non-enzymatical isolating technique was studied on the basis of structural observation of their ovules. The ovules, fresh and fixed by alcohol solution of P. suffruticosa were used to observe its structure characteristics with the whole clearing technique by 84 disinfectants and the slicing technique under a fluorescence microscope, and their fresh ovules were still used to isolate its nucellus and other structures with gum block under a stereomicroscope. Results identified the outer integument of P. suffruticosa ovules was extremely thick and easy to be peeled off. The outer layer of the inner integument of P. suffruticosa could be separated and isolated easily from the integument tapetum. In whole ovule only the integument tapetum could emit clear fluorescence. After the outer integument, the outer layer of the inner integument and the integument tapetum were peeled off successively with gum block under a stereomicroscope, the nucellus with original form could be isolated.