C、D型二价产气荚膜梭菌抗毒素血清的制备及保护效果评价

为治疗产气荚膜梭菌感染引起的疾病,研究制备了产气荚膜梭菌多价高效抗毒素血清。试验采用C、D型产气荚膜梭菌标准菌株,制备了高浓度外毒素和灭活疫苗,作为免疫原多次免疫绵羊,通过间接ELISA法监测绵羊抗体水平变化,采用小鼠中和试验检验绵羊抗毒素血清保护效果。结果表明:制备的高效价抗C、D型产气荚膜梭菌毒素血清每0.1 m L血清能中和400个C型毒素对小鼠的MLD和600个D型毒素MLD,有良好的应用前景。

Preparation and Evaluation of Bivalent Antisera of Clostridium perfringens Type C and D

High effect antiserum was prepared to treat infection caused by Clostridium perfringens.An high concentration of exotoxin was produced by standard strains of Clostridium perfringens type C and D, and used to prepare the inactivated vaccine. High effective antibody was obtained by immunizing sheep with the exotoxin and inactivated vaccine repeatedly. Indirect ELISA was used to monitor antibody level of the immunized sheep, and neutralization test in mice was used to detect protective effect of the serum. The results showed that 0.1 mL high potency antisera can neutralize 400 units MLD of type C and 600 units of type D in mice. It proved that the prepared antisera had a good application prospect.