| HSV-2对血管内皮样细胞ECV304的致病特性 |
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| 引用本文： | 赵海全,马文丽,危敏,柯昌文,郑焕英,郑文岭. HSV-2对血管内皮样细胞ECV304的致病特性[J]. 华中农业大学学报, 2008, 27(2): 267-272 |
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| 作者姓名： | 赵海全 马文丽 危敏 柯昌文 郑焕英 郑文岭 |
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| 作者单位： | 佛山科学技术学院生命科学学院,佛山,528231;南方医科大学基因工程研究所,广州,510515;广东省疾病预防与控制中心微生物检验所,广州,510300 |
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| 基金项目： | 广东省自然科学基金 , 广东省农业科技攻关项目 |
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| 摘 要： | 为了探讨HSV-2对血管内皮样细胞ECV304的致病特性,通过体外培养血管内皮样细胞ECV304并感染HSV-2后,采用RT-PCR和间接免疫荧光法检测HSV-2病毒的潜伏相关转录体基因的表达,并利用相差显微镜及电子显微镜观察ECV304感染HSV-2后的细胞形态变化.结果表明,血管内皮样细胞ECV304感染HSV-2后12～24 h即可检测到潜伏相关转录体基因表达;感染后1～2 d即可出现明显的细胞病变,提示HSV-2可感染ECV304细胞,并在其中活化复制,诱导ECV304细胞出现细胞融合和破裂死亡.
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| 关 键 词： | 单纯疱疹病毒2型 血管内皮样细胞ECV304 细胞病变 细胞融合 |
Cytopathic Effect of ECV304 Endothelial-like Cells Infected with Herpes Simplex Virus Type 2 |
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| ZHAO Hai-quan,MA Wen-li,WEI Min,KE Chang-wen,ZHENG Huan-ying,ZHENG Wen-ling. Cytopathic Effect of ECV304 Endothelial-like Cells Infected with Herpes Simplex Virus Type 2[J]. Journal of Huazhong Agricultural University, 2008, 27(2): 267-272 |
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| Authors: | ZHAO Hai-quan MA Wen-li WEI Min KE Chang-wen ZHENG Huan-ying ZHENG Wen-ling |
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| Abstract: | To investigate the mechanisms for cytopathic effect(CPE)of herpes simplex virus type 2(HSV-2)in ECV304 endothelial-like cells,RT-PCR and indirect immunofluorescence were used to detect HSV-2 infection.Phase-contrast and electron microscopy were performed to analyze morphology changes in infected and uninfected groups.In HSV-2-infected ECV304 cells,cytopathic effects were first observed approximately at 1-2 days post-infection.After 12-24 h infection,the majority of cells with CPE changes exhibited detachmen... |
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| Keywords: | herpes simplex virus type 2 ECV304 endothelial-like cells cytopathic effect cell fusion |
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