大黄鱼刺激隐核虫病继发细菌感染致死原因的研究

为明确大黄鱼刺激隐虫病大量死亡的致死原因,从患刺激隐核虫（Cryptocaryon irritans）病的大黄鱼（Pseudosciaena crocea）肝脏中分离细菌,病情程度较轻的鱼未分离到细菌,非常严重的鱼分离到细菌;分离的菌株经纯化后鉴定为创伤弧菌（Vibrio vulnificus）,利用纯化后的菌株作回归感染试验,其感染率为100%,死亡率为80%。研究表明患刺激隐核虫病的大黄鱼体内的细菌为继发性感染,是导致大黄鱼大量死亡的致病菌,其感染途径为水→伤口→体内。

Research on death caused by secondary bacterial infection of Cryptocaryon irritans on Pseudosciaena crocea

The research separated bacteria from livers of Pseudosciaena crocea suffering from Cryptocaryon irritans,and the result showed that bacteria couldn’t be separated from Pseudosciaena crocea with relatively light disease,but could be separated with severe disease.Separated virus was tested after purifying as Vibrio vulnificus.Regression infection experiment of Vibrio vulnificus demonstrated that the morbidity and mortality were 100% and 80%,respectively.The research showed that the bacteria in Pseudosciaena crocea suffering from Cryptocaryon irritans were secondary infection.It was a nosogenetic virus causing the death of Pseudosciaena crocea.Its infection way was from water to vulnificus to bodies.