| 胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留 |
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| 引用本文： | 吴茂生,何方洋,赵正苗,汪善良,崔廷婷,刘婷婷.胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留[J].福建水产,2012,34(4):290-295. |
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| 作者姓名： | 吴茂生 何方洋 赵正苗 汪善良 崔廷婷 刘婷婷 |
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| 作者单位： | 1. 福建省海洋环境与渔业资源监测中心,福建福州,350003
2. 北京勤邦生物技术有限公司,北京,102206 |
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| 摘 要： | 采用胶体金免疫层析技术(GICA)测定水产品中的呋喃妥因代谢物残留,并用液相色谱-串联质谱(LC-MS/MS)对实验结果进行确证。结果显示,胶体金免疫层析法的检测限为1.0μg/kg,假阳性率不大于5%,假阴性率为0;对实际样本的检测结果与LC-MS/MS法一致。研究表明,胶体金试纸条法操作简便、快速,成本低,为水产品中呋喃妥因代谢物的残留检测提供了快速筛选方法,而LC-MS/MS法灵敏度高,结果准确,适用于阳性样本的确证和精确定量。
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| 关 键 词： | 呋喃妥因代谢物 水产品 胶体金免疫层析法(GICA) 液相色谱-串联质谱法(LC-MS/MS) |
Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products |
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| WU Mao-sheng,HE Fang-yang,ZHAO Zheng-miao,WANG Shan-liang,CUI Ting-ting,LIU Ting-ting.Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products[J].Journal of Fujian Fisheries,2012,34(4):290-295. |
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| Authors: | WU Mao-sheng HE Fang-yang ZHAO Zheng-miao WANG Shan-liang CUI Ting-ting LIU Ting-ting |
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| Institution: | 1. Ocean Environment and Fishery Resource Monitoring Center of Fujian Province, Fuzhou 350003, China; 2. Beijing Kwinbon Biotechnology Co. Ltd. , Beijing 102206, China) |
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| Abstract: | Gold immunochromatographic assay （GICA） was used to determine the nitrofurantoin metabolite （AHD） residues in aquatic products, and also the liquid chromatographytandem mass spectrometry （LC - MS/MS） was used to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5 % , and false negative rate was 0. The results of the two methods for aquatic products were consistent. The results showed that GICA had the advantages of convenient operation, provided a rapid method for the screen of AHD in aquatic products, while LC - able for determination of positive samples. rapidity and low cost, which MS/MS was accurate and suit able for determination of positive samples. |
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| Keywords: | nitrofurantoin metabolite (AHD) aquatic products gold immunochromatographic assay ( GICA) liquid chromatographytandem mass spectrometric (LC- MS/MS) |
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