| 活性污泥胞外聚合物提取条件的优化(英文) |
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| 引用本文： | 唐金花,许国仁,萧静,Ludovico Spinosa,李圭白. 活性污泥胞外聚合物提取条件的优化(英文)[J]. 农业科学与技术, 2012, 0(2): 384-387,433 |
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| 作者姓名： | 唐金花 许国仁 萧静 Ludovico Spinosa 李圭白 |
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| 作者单位： | 哈尔滨工业大学;黑龙江东方学院;巴里技术大学 |
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| 基金项目： | Supported by the National High-tech Research and Develop Program of China("863"Program)(2009AA064704);the National Natural Science Foundation of China(51038003);the Program for New Century Excellent Talents in University by the State Education Ministry(NCET-08-161)~~ |
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| 摘 要： | [目的]探索活性污泥胞外聚合物(EPS)提取的最佳条件。[方法]比较研究了5种方法(H2SO4法、甲醛-NaOH法、搅拌法、热提法和NaOH法)对活性污泥EPS的提取效果,同时确定了最适提取方法的最优提取条件。[结果]5种提取方法中NaOH法因其提取的DNA含量较少,提取时间较短进而比较适宜。以NaOH法提取EPS,在pH为11,提取时间为10min,搅拌速度在80~120r/min下提取效率最高。[结论]确定的EPS最适提取条件可为其研究提供理论基础。
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| 关 键 词： | 活性污泥 胞外聚合物 提取 |
Optimization of Extraction Conditions of Extracellular Polymeric Substances from Activated Sludge |
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| Jinhua TANG,Guoren XU,Jing XIAO,Ludovico Spinosa,Guibai LI. Optimization of Extraction Conditions of Extracellular Polymeric Substances from Activated Sludge[J]. Agricultural Science ＆ Technology, 2012, 0(2): 384-387,433 |
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| Authors: | Jinhua TANG Guoren XU Jing XIAO Ludovico Spinosa Guibai LI |
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| Affiliation: | 1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China; 2. East University of Heilongjiang, Harbin 150001, China; 3. Technical University of Bari, Taranto 74100, Italy |
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| Abstract: | [Objective] To explore the optimal extraction conditions of extracellular polymeric substances (EPS) from activated sludge. [Method] The efficiency of five methods (H2SO4, formaldehyde-NaOH, mixing, heating and NaOH) on the extraction of EPS was investigated comparatively. The optimal extraction conditions of the most suitable method were determined. [Result] NaOH method is most effective in extracting EPS with less DNA contamination and shortened extraction period. The optimal extraction condition was pH of 11, extraction time of 10 min and agitation speed of 80-120 r/min. [Conclusion] The determined optimal extraction condition provided theoretical basis for EPS study. |
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| Keywords: | Activated sludge Extracellular polymeric substances (EPS) Extraction |
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