| 卡那霉素对杨树和刺槐外植体生长与分化的影响 |
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| 引用本文： | 郑进,康薇,王慧,刘凯于,洪华珠,彭建新.卡那霉素对杨树和刺槐外植体生长与分化的影响[J].核农学报,2006,20(2):116-117,131. |
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| 作者姓名： | 郑进 康薇 王慧 刘凯于 洪华珠 彭建新 |
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| 作者单位： | 华中师范大学昆虫学研究所,湖北,武汉,430079;湖北生态工程职业技术学院,湖北,武汉,430200;华中师范大学昆虫学研究所,湖北,武汉,430079 |
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| 基金项目： | 湖北省林业厅重点科技基金 |
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| 摘 要： | 卡那霉素(Km)对中嘉8号杨芽苗生长具有明显的抑制作用,随着浓度的提高,芽苗存活率逐渐降低,当Km≥60 mg/L时,存活率为0。Km对宽叶刺槐茎段的分化和芽的生长有较大影响,Km=10 mg/L时,促进分化,分化频率达100%,高出对照11.1%;Km≥40mg/L时,抑制芽的生长,芽的黄化率为100%;Km≥60mg/L时,没有芽的分化。因此,中嘉8号杨、宽叶刺槐遗传转化用卡那霉素为选择剂是合适的。
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| 关 键 词： | 杨树 刺槐 组织培养 抗生素 |
| 文章编号： | 1000-8551（2006）02-116-02 |
| 收稿时间： | 2005-05-15 |
| 修稿时间： | 2005-05-15 |
EFFECTS OF KANAMYCIN ON THE GROWTH AND DETERMINATION OF POPLA AND BLACUST |
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| ZHENG Jin,KANG Wei,WANG Hui,LIU Kai-yu,HONG Hua-zhu,PENG Jian-xin.EFFECTS OF KANAMYCIN ON THE GROWTH AND DETERMINATION OF POPLA AND BLACUST[J].Acta Agriculturae Nucleatae Sinica,2006,20(2):116-117,131. |
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| Authors: | ZHENG Jin KANG Wei WANG Hui LIU Kai-yu HONG Hua-zhu PENG Jian-xin |
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| Institution: | 1, Institute of Entomology, Central China Normal University, Wuhan , Hubei 430079 ;2. Hubei Ecology Vocational College, Wuhan, Hubei 430200 |
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| Abstract: | Kanamycin(Km) showed inhibition effects on the growth of Populus deltoids "63/69" with plant survival rates being reduced as concentration increesing.When Km≥60 mg/L,there was no plant survived.It also had inhibition effects on the regeneration and growth of shoots of Robinia pseudoacacia L.When Km=10 mg/L,the shoots regenerated were promoted and shoots induction rates was 100%,11.1% over control.When Km≥40 mg/L,shoots etiolation rates was 100%.When Km=60 mg/L,there was no shoots regenerated.Thus,Km is optimal for genetic engineering of the "63/69" and Robinia pseudoacacia L. |
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| Keywords: | Populus deltoides Robinia pseudoacacia L tissue culture antibiotics |
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