| 中国美利奴羊IGFBP-3基因内含子4区的PCR-RFLP多态性分析 |
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| 引用本文： | 沈敏,王文君,杨永林,马春萍,刘守仁,李宁.中国美利奴羊IGFBP-3基因内含子4区的PCR-RFLP多态性分析[J].中国畜牧兽医,2010,37(8):134-137. |
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| 作者姓名： | 沈敏 王文君 杨永林 马春萍 刘守仁 李宁 |
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| 作者单位： | （1.新疆农垦科学院兵团绵羊繁育生物技术重点实验室，石河子 832000；2.江西农业大学食品科学与工程学院，南昌 330045；3.新疆紫泥泉绵羊育种研究所，石河子 832025；4.中国农业大学农业生物技术国家重点实验室，北京 100193） |
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| 摘 要： | 本研究针对在绵羊IGFBP-3基因内含子4区新发现的1个SNP位点(C218T)，建立了PCR-RFLP的基因分型方法。利用该方法对353只中国美利奴羊(新疆军垦型)进行了多态性检测，结果显示，有CC（249 bp）、CT（249/216/33 bp）和TT（216/33 bp）3种基因型，基因型频率分别为0.52、0.41和0.07；有C、T 2种等位基因，等位基因频率分别为0.72、0.28。多重比较结果表明，不同基因型对中国美利奴部分羊毛生产性能有一定的影响，CC基因型个体的毛丛长度显著短于TT基因型个体(P＜0.05)，其毛囊密度显著高于CT和TT基因型个体(P＜0.05)。
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| 关 键 词： | 胰岛素样生长因子结合蛋白3 单核苷酸多态性 羊毛性状 中国美利奴羊 |
PCR-RFLP Polymorphism in Intron 4 of IGFBP-3 Gene in Chinese Merino Sheep |
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| SHEN Min,WANG Wen-Jun,YANG Yong-Lin,MA Chun-ping,LIU Shou-ren,LI Ning.PCR-RFLP Polymorphism in Intron 4 of IGFBP-3 Gene in Chinese Merino Sheep[J].China Animal Husbandry & Veterinary Medicine,2010,37(8):134-137. |
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| Authors: | SHEN Min WANG Wen-Jun YANG Yong-Lin MA Chun-ping LIU Shou-ren LI Ning |
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| Institution: | （1.Key Laboratory for Biotechnology of Sheep Breeding of Xinjiang Production & Construction Corps，Xinjiang Academy of Agricultural Reclamation，Shihezi 832000，China；2.College of Food Science and Engineering，Jiangxi Agricultural University，Nanchang 330045，China；3.Ziniquan Research Institute of Sheep Breeding，Shihezi 832025，China；4.State Key Laboratory for Agrobiotechnology，China Agricultural University，Beijing 100193，China） |
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| Abstract: | In this study, PCR-RFLP method was established according to a novel SNP site (C218T) within the intron 4 of sheep insulin-like growth factor binding proteins-3 （IGFBP-3） gene. Using this method, 353 Chinese Merino sheeps (Xinjiang Junken type) were tested and illustrated three unique binding genotypes of CC (249 bp), CT (249/216/33 bp) and TT (216/33bp). Frequencies of the genotype CC, CT, TT were 0.52, 0.42, 0.07, respectively, and frequencies of the allele C, T were 0.72, 0.28, respectively. Different genotypes slightly affected several wool traits of Chinese Merino sheeps. Staple length (SL) in individuals of genotype CC was significantly shorter than that in individuals of genotype TT (P＜0.05), follicle density in individuals of genotype CC was significantly higher than that in individuals of genotype CT and TT (P＜0.05). |
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| Keywords: | insulin-like growth factor binding proteins-3 single nucleotide polymorphism wool trait Chinese Merino sheep |
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