Specific expression of a sesame storage protein in transgenic rice bran |
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| Affiliation: | 1. Graduate Institute of Biotechnology, National Chung-Hsing University, 250 Kuo-Kuang Road, Taichung 40227, Taiwan, ROC;2. Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, ROC;3. Department of Agronomy, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC;4. Institute of Molecular Biology, National Chung-Hsing University, Taichung, Taiwan, ROC;1. Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina;2. Consejo Nacional de Investigación Científica y Técnicas de la República Argentina (CONICET), Argentina;1. Department of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria;2. Department of Botany, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria;3. AgroBioInstitute, Buld. Dragan Tzankov 8, 1164 Sofia, Bulgaria;4. Department of Biophysics, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria;1. Division of Surgery, Assaf Harofeh Medical Center, Zerifin, affiliated to Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel;2. Department of Plastic Surgery, Assaf Harofeh Medical Center, Zerifin, affiliated to Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel;1. Division of Nutrition, National Food Institute, Technical University of Denmark, Søborg, Denmark;2. Research Centre for Prevention and Health, The Capital Region of Denmark, Glostrup, Denmark;3. Faculty of Health Science, University of Copenhagen, Denmark;4. Faculty of Medicine, University of Aalborg, Aalborg, Denmark;5. Department of Endocrinology and Gastroenterology, Bispebjerg University Hospital, Copenhagen, Denmark;6. Department of Endocrinology and Medicine, Aalborg University Hospital, Aalborg, Denmark;7. Department of Gastroenterology, Slagelse Hospital, Slagelse, Denmark;1. Polymères, Colloïds, Interfaces, UMR CNRS Université du Maine, 72085 Le Mans Cedex 9, France;2. BioPol, Chemistry Department, Federal University of Paraná, 81531-980 Curitiba, PR, Brazil;3. Université de Tunis El Manar, Faculté des Sciences de Tunis, LR99ES16 Laboratoire Physique de la Matière Molle et dela Modélisation Électromagnétique, 2092, Tunis, Tunisia |
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| Abstract: | Rice oil bodies enclosed by unique structural proteins, oleosins, are found in the embryo and the aleurone layer, but not the starchy endosperm where starch and storage proteins are accumulated. To examine oleosin promoter specificity, a sesame storage protein, 2S albumin, was expressed in transgenic rice seeds under the control of a rice oleosin promoter. In all transgenic rice seeds, the sesame 2S albumin was found exclusively in the bran fraction after milling. Immunological staining revealed that the sesame 2S albumin was also located in the embryo and the outermost cells of the starchy endosperm. Furthermore, immunogold labeling showed that the transgenic 2S albumin was deposited in both type-I and type-II protein bodies of the outermost cells of the endosperm as well as in the type-II protein bodies of the embryo. The methionine and cysteine contents in the bran from four homozygous transgenic lines were elevated by 24–38 and 50–62%, respectively, compared with those of wild-type plants. The results suggest that the rice oleosin promoter is bran-specific and could be used to add value to rice bran, an abundant by-product of rice polishing, by genetic engineering. |
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