A new puroindoline b mutation present in Chinese winter wheat cultivar Jingdong 11 |
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| Affiliation: | 1. Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), No. 12, Zhongguancun South Street, Beijing 100081, China;2. CIMMYT China Office, c/o CAAS, No. 12, Zhongguancun South Street, Beijing 100081, China;3. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, DF, Mexico;4. USDA-ARS Western Wheat Quality Laboratory, P.O. Box 646394, Washington State University, Pullman, WA 99164-6394, USA;1. Consejo Nacional de lnvestigaciones Científicas y Técnicas (CONICET), Instituto de Recursos Biológicos, Instituto Nacional de Tecnología Agropecuaria (INTA), Villa Udaondo, 1686, Hurlingham, Buenos Aires, Argentina;2. Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Genética, Buenos Aires, Argentina;3. Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, 80523, USA;4. CONICET - Instituto de Suelos, INTA, Villa Udaondo, 1686, Hurlingham, Buenos Aires, Argentina;1. Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, India;2. Division of Genetics, Indian Agricultural Research Institute, New Delhi 110012, India |
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| Abstract: | Kernel hardness is one of the most important characteristics in determining utilization and marketing of bread wheat. Genes coding for puroindoline a and b (PINA and PINB) were located at the Ha locus and designated as Pina-D1 and Pinb-D1, respectively. The coding sequence of the Pinb gene in a Chinese winter wheat cultivar Jingdong 11 (Triticum aestivum L.) was amplified with polymerase chain reaction (PCR), and the obtained 447-bp fragment sequenced from two strands, and compared with the eight known Pinb alleles. The results showed that Jingdong 11 possessed a new Pinb allele not reported previously, and was designated as Pinb-D1q. It is characterized by a single base T to G substitution, which results in a tryptophan to leucine substitution (TGG to TTG) at position 44 and is most likely the cause of hard grain texture in Jingdong 11. The characterization of Pinb-D1 alleles would be helpful in manipulating grain hardness of bread wheat in breeding programs. |
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