A new <Emphasis Type="Italic">Rhizoctonia</Emphasis> sp. closely related to <Emphasis Type="Italic">Waitea circinata</Emphasis> causes a new disease of creeping bentgrass |
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Authors: | Takeshi Toda Toshihiro Hayakawa Joseph Mwafaida Mghalu Shigeharu Yaguchi Mitsuro Hyakumachi |
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Institution: | (1) Laboratory of Plant Pathology, Faculty of Applied Biological Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;(2) Research Institute of Riken Green Co., Ltd, 859-1 Minamidaiheshinden, Iwata Shizuoka, 437-1218, Japan;(3) The United Graduate School of Agricultural Science, Gifu university, 1-1 Yanagido, Gifu 501-1193, Japan;(4) Present address: Department of Plant Pathology, North Carolina State University, Center for Fungal Research, Campus Box 7521, Raleigh, NC 27695, USA |
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Abstract: | Isolates of an unidentified Rhizoctonia sp. (UR isolates) were obtained from creeping bentgrass and Kentucky bluegrass with reddish brown sheath and foliar rots.
Because the UR isolates anastomosed with isolates of three varieties of Waitea circinata (var. oryzae, var. zeae, and var. circinata), colony morphology, hyphal growth rate at different temperatures, pathogenicity, sequence analysis of the internal transcribed
spacers (ITS) region of ribosomal RNA genes (rDNA) were compared. The colony color of mature UR isolates was distinct from
isolates of the other three varieties of W. circinata. In pathogenicity tests on creeping bentgrass, the severity of the disease caused by UR isolates was significantly higher
than that caused by the three varieties of W. circinata. Sequence similarities of the rDNA-ITS region between UR isolates and between isolates within each variety were high (97–100%),
but they were lower among isolates from UR and the varieties of W. circinata (88–94%). In a phylogenetic tree based on the rDNA-ITS sequences, UR isolates formed a cluster separate from each of the
clusters formed by the three varieties of W. circinata. These results indicate that the UR isolates clearly differ from the three varieties of W. circinata. We therefore propose that the UR isolates be classified as new Rhizoctonia sp. that are closely related to W.
circinata and that the disease on creeping bentgrass should be called Waitea reddish-brown patch disease (Sekikasshoku-hagusare-byo
in Japanese). |
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Keywords: | Waitea circinata Rhizoctonia oryzae Rhizoctonia zeae Agrostis stolonifera var palustris Poa pratensis |
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