马尾松胚胎发育的观察研究

在自然条件下，马尾松成熟花粉粒发育需6个月，授粉有效期在珠鳞展开的头5天，授粉后雌配子体游离核时期持续12个月，从游离核到细胞约需7天，颈卵器2－3个，受精作用发生在授粉后第13个月，原胚期约30天，幼胚期约40，成熟胚期约60天；从球花芽分化到种子成熟约需24个月，在形态发育的观察中，发现低温冻害会引起花粉在发育各阶段的形态异常及大量败育。

OBSERVATION ON EMBRYO DEVELOPMENT OF MASSON PINE

Under natural condition, the development of a mature pollen took 6 months to complete. The first five days after ovuliferous scale open were of efficiency for pollination. The phase of free nuclei of female gametophyte lasted for 12 months, and it took about 7 days to develop from nuclei phase to cell phase. There were two or three archegoniums in Masson Pine. The fertilization took place in the 13th month after pollination. The development phase of proembryo was about 30 days, young embryo 40 days and mature embryo 60 days. The production of mature seeds needed 24 months to go since the differentiation of floral buds. According to the observation on morphological development, we found that there usually were unnormal pollens and pollen sterility in every developing stage because of low temperature and freezing.