First derivative spectrophotometric determination of anhydrotetracyclines in tetracyclines |
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| Authors: | A A Wahbi H A Al-Khamees A M Yousef |
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| Affiliation: | King Saud University, College of Pharmacy, Pharmaceutical Chemistry Department, Riyadh, Saudi Arabia. |
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| Abstract: | A rapid method is presented for detection and determination of anhydrotetracycline-HCl (ATC-HCl) and 4-epianhydrotetracycline-HCl (4-EATC-HCl) in tetracycline-HCl (TC-HCl). The method determines the 2 compounds as a sum, not individually. The first derivative absorption curve has a trough (D1) at 460 nm which is linearly related to concentration (1-10 mg/mL). ATC-HCl + 4-EATC-HCl content was determined in TC-HCl powder and capsules by the D1 and the compensation D1 spectrophotometric methods. The results were compared with those obtained using U.S. Pharmacopeia and British Pharmacopoeia methods. |
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