One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9
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| Authors: | Wataru FUJII Asuka ONUMA Koji SUGIURA Kunihiko NAITO |
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| Institution: | 1)Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan |
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| Abstract: | Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications
on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations
on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes
simultaneously, and these mutations were normally inherited in the next generation. |
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| Keywords: | CRISPR/Cas9 Heritable Mouse Phenotype Triple knockout |
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