| Abstract: | The placenta is a unique pregnancy-related tissue and plays a key role in occurrence of unexplained recurrent pregnancy loss
(URPL). Abnormal placentation might play a key role in occurrence of URPL. Therefore, the purpose of this study was to compare the
human placental proteome between URPL placentas and normal placental matched for gestational week. Total placental proteins were
extracted, and the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was used for separation of the placental
proteomes. Protein spots differentially expressed between URPL and normal placentas were selected and identified by the
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF) technique after being digested in the
gel. Moreover, quantitative real-time PCR and Western blot techniques were used to confirm the differential expression mass
results for some differentially expressed proteins. The results indicated that at least 19 protein spots were differentially
expressed between URPL and normal placentas (P < 0.05), and twelve of them were successfully identified. While only two
proteins were downregulated (calumenin and enolase 1), the remaining ten spots (actin gamma 1 propeptide, cathepsin D
prepropeptide, heat shock protein gp96, tubulin beta, tubulin alpha 1, glutathione S-transferase, vitamin D binding protein,
prohibitin, actin beta, apolipoprotein A-I) showed increased expression in URPL cases in comparison with normal placentas.
Real-time PCR also confirmed the downregulation of calumenin and upregulation of prohibitin and apolipoprotein A-I at the mRNA
levels. In conclusion, the results of the present study showed that alteration in the expression of proteins involved in
proliferation and migration of endothelial cells as well as control of coagulation by these cells might play an important role in
the pathogenesis of URPL. |
