| 在COS细胞中表达白细胞介素-Ⅱ |
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| 引用本文： | 周台英,张鹤龄,王秀梅,博晓真,郭荧,高凯. 在COS细胞中表达白细胞介素-Ⅱ[J]. 内蒙古农业大学学报(自然科学版), 2007, 28(1): 192-194 |
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| 作者姓名： | 周台英 张鹤龄 王秀梅 博晓真 郭荧 高凯 |
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| 作者单位： | 内蒙古医学院研究生学院,呼和浩特,010059;内蒙古大学生命科学院,呼和浩特,010021;中国药品生物制品检定所,北京,100050 |
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| 摘 要： | 为了进一步证实IL-2的存在,我们对IL-2真核表达载体进行了pC-IL-2双酶切鉴定。采用DEAE-Dextran介导的方法,用IL-2真核表达载体pC-IL-2转染COS细胞进行暂态表达。经放射免疫测定了转染细胞上清中IL-2表达量为46.05ng/m l。采用依赖IL-2的CTLL-2细胞作为靶细胞的MTT法测定了IL-2表达产物的活性为86 IU/m l。
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| 关 键 词： | COS细胞 IL-2表达 放免分析 |
| 文章编号： | 1009-3575（2007）01-0192-03 |
| 修稿时间： | 2006-10-09 |
EXPRESSION OF IL-2 IN COS CELL |
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| ZHOU Tai-ying,ZHANG he-ling,WANG Xiu-mei,BO Xiao-zhen,GUO Ying,GAO Kai. EXPRESSION OF IL-2 IN COS CELL[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition), 2007, 28(1): 192-194 |
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| Authors: | ZHOU Tai-ying ZHANG he-ling WANG Xiu-mei BO Xiao-zhen GUO Ying GAO Kai |
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| Affiliation: | 1. Department of Gradnate, Inner Mongolia Medical College, Hohhot 010059,China ; 2. College of Life Sciences; Inner Mongolia University Hohhot 010021 ,China; 3. National Institute for the Control of Pharmaceutical and Biological Products,Beifing 100050, China |
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| Abstract: | To confirm the insert LI-2 the recombinant plasmids PC-IL-2 was digested by EcoRI/BamHI.The COS cells were transinfected by pC-IL-2 using DEAE-Dextran method for transient expression of IL-2 gene.The concentration of expressed IL-2 was up to 46.05ng/ml.In supernatant of the transinfected COS cells the activity of expressed IL-2 was 86 IU/ml. |
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| Keywords: | COS cell expression of IL-2 radioimmunoassay CLC number |
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