| 长春碱纳米粒的制备及其抗肿瘤活性 |
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| 引用本文： | 刘玉梅,欧阳五庆,马淑燕,杨宝平.长春碱纳米粒的制备及其抗肿瘤活性[J].上海交通大学学报(农业科学版),2008,26(3):228-232. |
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| 作者姓名： | 刘玉梅 欧阳五庆 马淑燕 杨宝平 |
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| 作者单位： | 1. 西北农林科技大学,动物医学院,杨凌,712100;河南科技大学,动物科技学院,洛阳,471003
2. 西北农林科技大学,动物医学院,杨凌,712100 |
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| 摘 要： | 以右旋糖苷为稳定剂，通过乳化聚合法制备长春碱聚氰基丙烯酸正丁酯纳米粒，检测其基本性质（形态、粒径分布、包封率和载药量）。通过MTT法检测长春碱纳米粒对神经胶质瘤细胞BT325的细胞毒性作用、经倒置显微镜观察和HE染色，探讨了长春碱纳米粒对神经胶质瘤细胞BT325形态的影响。结果显示，长春碱纳米粒平均粒径为74．4am，包封率为78．47％，载药量为39．24％。与长春碱原料药组相比，长春碱纳米粒组细胞吸光度显著降低，细胞数量明显减少，凋亡细胞增多。聚氰基丙烯酸丁酯纳米粒可作为长春碱进入胶贡瘤细胞的有效载体。
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| 关 键 词： | 长春碱 聚氰基丙烯酸正丁酯纳米粒 神经胶质瘤 |
Preparation of Vinblastine Nanoparticles and Its Anti-tumor Effect |
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| LIU Yu-mei,OUYANG Wu-qing,MA Shu-yan,YANG Bao-ping.Preparation of Vinblastine Nanoparticles and Its Anti-tumor Effect[J].Journal of Shanghai Jiaotong University (Agricultural Science),2008,26(3):228-232. |
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| Authors: | LIU Yu-mei OUYANG Wu-qing MA Shu-yan YANG Bao-ping |
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| Institution: | LIU Yu-mei 1,2,OUYANG Wu-qing 1,MA Shu-yan1,YANG Bao-ping1 (1. College of Veterinary Medicine,Northwest Agriculture , Forestry University,Yangling 712100,China,2. College of Animal Science , Technology,Henan University of Science , Technology,Luoyang 471003,China) |
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| Abstract: | Vinblastine loaded poly(buty1 cyanoacrylate) nanoparticles(VLB-PBCA-NP) were prepared by emulsion polymerization process and using dextran as a stabilizing agent. Vinblastine nanoparticles were characterized by means of morphology, size, drug entrapment efficiency and loading efficiency. VLB-PBCA-NP cytotoxicity profile was monitored using BT325 glioma cells by MTT assay. Morphological character was observed by inverted microscope and HE staining. The results showed that mean diameter of VLB-PBCA-NP was abo... |
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| Keywords: | vinblastine PBCA-NP glioma |
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