Genetic characterization and identification of new accessions from Syria in an olive germplasm bank by means of RAPD markers |
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Authors: | Angjelina Belaj Juan M Caballero Diego Barranco Luis Rallo Isabel Trujillo |
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Institution: | (1) Departamento de Agronomía, ETSIAM, Universidad de Cordoba, Avenida Ménendez Pidal s/n, Apdo 3048, 14080 Cordoba, Spain;(2) Departamento de Olivicultura y Elaiotecnia, Centro de Investigación y Formación Agraria (CIFA) `Alameda del Obispo', 14080 Cordoba, Spain |
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Abstract: | Thirty-two olive cultivar accessions from Syria, most of them obtained from collecting expeditions, were characterized by
means of RAPD markers before being introduced in the World Germplasm Bank of Cordoba. A total of 79 polymorphic bands(6.1
polymorphisms per primer) out of 93(7.1 bands per primer) were scored for the13 primers used, corresponding to 84.9% of the
amplification products. Thirty-one different genotypes were clearly discriminated. Differences were not found among the amplification
profiles from different individuals of the same cultivar. Only two cases of mislabeling or errors of planting were found.
Fourteen accessions corresponding to 6 homonyms were discriminated by RAPDs as different genotypes. The dendrogram obtained
by RAPD analysis included three major groups. Some evidence of relationships of the Syrian accessions studied according to
their geographic origin and/or diffusion was found. For instance, cultivars from the Central Syria (Tadmur/Palmyra)such as
Toffahi', ‘Abbadi Abo Gabra’-1033,‘Abo Kanani’, ‘Shami’-1041, ‘Abbadi Shalal’ ‘Adgam’-844 and ‘Majhol’-1013 clustered in Group
1 and 2. Six cultivars from Northern Syria clustered in Group 2. But it was not found a geographic structure for the cultivars
from South and West of Syria. These results agree with the hypothesis of autochthonous origin of most of the olive cultivars.
Some associations between cultivars from Central Syria and their fruit size were observed. This suggests that fruit size was
a criterion of local selection in olive cultivars of this area.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | collection genetic resources identification molecular markers Olea europaea L |
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