18个早熟禾品种SSR指纹图谱的构建

利用从草地早熟禾(Poa pratensis)基因组中开发出来的3对多态性SSR引物,对18个早熟禾品种进行分子指纹图谱鉴别研究。结果表明:3对引物在18个品种中共检测出16个多态性片段,每对引物可以检测到4至6个数目不等的片段,平均为5.33个,片段大小介于152至227bp。利用单一引物可将18个品种区分为5~11种类型,平均为8.33个类型。利用这3对SSR多态性引物指纹图谱可以将18个品种完全区分开。研究构建的指纹图谱可作为对文中18个早熟禾品种进行区分的重要参考依据,同时筛选出的对核心引物也可以用来构建其他早熟禾品种的指纹图谱。

Fingerprint establishment of 18 Poa pratensis cultivars with SSR markers

The fingerprint of 18 Poa pratensis cultivars was established by using 3 polymorphic simple se-quences repeat (SSR)markers developed from genomic DNA of P .pratensis .Totally 1 6 polymorphic SSR fragments were detected in all tested cultivars,and 4 to 6 alleles were detected by each pair of SSR primer with an average of 5.33.The band size varied from 152 bp to 227 bp.18 cultivars could be divided into 5 to 1 1 groups by single polymorphism primer,with an average of 8.33.18 cultivars could be distinguished thoroughly by the fingerprinting maps constructed by the 3 pairs of SSR primers.The constructed fingerprinting maps are important references to discriminate the 18 tested cultivars.Furthermore,the screened 3 polymorphic SSR markers can be useful to establish fingerprint for other cultivars.