方斑东风螺地膜覆沙池和潮线下养殖系统细菌种群结构的PCR-DGGE 分析

为了解方斑东风螺养殖系统中细菌种群结构,研究和优化方斑东风螺地膜覆沙池养殖体系的管理模式。试验采用PCR-DGGE技术,对方斑东风螺地膜覆沙池养殖系统和潮线下养殖系统底质样品和水体样品中的细菌种群结构进行了分析。试验结果显示,以广东省湛江市东海岛海水为基础构建的方斑东风螺养殖系统内主要细菌种群包括变形菌门、绿弯菌门、蓝细菌门和放线菌门。其中地膜覆沙池养殖系统主要细菌种群包括γ-变形菌纲、α-变形菌纲、δ-变形菌纲、变形菌纲、厌氧绳菌纲、蓝细菌和放线菌等;潮线下养殖系统主要细菌种群包括γ-变形菌纲、α-变形菌纲、δ-变形菌纲、厌氧绳菌纲、蓝细菌,而变形菌纲和放线菌的分布较少。被检测的两个养殖系统中细菌种群均具有较高的多样性,UPGMA聚类分析结果表明,被检测的两个养殖系统底质样品S1和S2为一簇,种群结构相似度为54.5%;两个养殖系统的水体样品S3和S4为一簇,种群结构相似度为84.0%;试验结果说明,通过池塘水体更新和微生物种群监测等调控手段有效控制养殖系统微生物种群结构,能够使方斑东风螺地膜覆沙池养殖系统的微生物生态水平接近潮线下养殖系统。

PCR-DGGE Analysis of Bacterial Communities Structure in Babylonia areolata Culture Systems of The Subtidal Zone and The Pond Mulched Plastic Film and Sand in Bottom

To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattern of Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom, the bacterial communities in Babylonia areolata culture systems of the subtidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the subtidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobacteria Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Epsilonproteobacteria, Anaerolineae, Cyanobacteria and Actinobacteria. The dominant bacterial communities in the subtidal zone culture system were Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteria, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture systems. The results of unweighted pair group method with arithmetic average (UPGMA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5% . The bacterial communities of seawater samples S3 and S4 in the above culture systems were a cluster, the similarity of the bacterial communities was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the subtidal zone culture systems through changing the pond seawater and monitoring the microbial population.