| 不同破眠处理对油桃休眠芽H2O2含量及 相关酶活性的影响 |
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| 引用本文： | 谭钺,王茂生,吕勐,崔海金.不同破眠处理对油桃休眠芽H2O2含量及 相关酶活性的影响[J].中国农学通报,2014,30(28):128-132. |
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| 作者姓名： | 谭钺 王茂生 吕勐 崔海金 |
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| 作者单位： | 山东省果树研究所,山东省莒南县望海楼国有林场,青岛市动物园,山东省果树研究所 |
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| 基金项目： | 山东省现代农业产业技术体系水果创新团队专项基金(SDAIT-03-022-04) |
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| 摘 要： | 为进一步探讨H2O2代谢与果树芽自然休眠解除的关系,检测了人工破眠过程中‘曙光’油桃芽内H2O2含量和过氧化氢酶（CAT）、过氧化物酶（POD）活性的影响。选用的人工破眠处理分别为50℃高温（HT）处理、单氰胺（HC）处理和TDZ（TDZ）处理。结果表明,50℃高温和单氰胺效果相似,都能显著打破芽深休眠,并且抑制芽内CAT活性,引起H2O2含量增加,但2个处理对POD活性的影响都不大。TDZ打破深休眠的效果较差,对芽内H2O2含量、CAT活性、POD活性均未表现出显著影响。CAT活性抑制和H2O2积累可能是50℃高温和单氰胺打破自然休眠作用机制的重要组成部分。
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| 关 键 词： | 破眠处理 H2O2代谢 芽休眠 油桃 |
| 收稿时间： | 5/8/2014 12:00:00 AM |
| 修稿时间： | 5/8/2014 12:00:00 AM |
Effects of Different Dormancy-breaking Treatments on H2O2 Content and Activity of Related Enzymes of Dormant Nectarine Buds |
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| Institution: | Tan Yue,Wang Maosheng,Lv Meng,Cui Haijin(1Shandong Institute of Pomology/ Shandong Key Lab of Fruit Tree Biotechnology Breeding, Tai＇an Shandong 271000; 2Wanghailou National Forest Farm of Junan, Linyi Shandong 276600; 3Qingdao Zoo, Qingdao Shandong 266003) |
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| Abstract: | In order to further explore relationships between bud dormancy release and H2O2 metabolism,changes of H2O2 level, CAT activity and POD activity of‘Shuguang＇nectarine buds during artificial dormancy-breaking process were studied. High temperature of 50℃（HT）, hydrogen cyanamide（HC） and TDZ（TDZ） were selected as dormancy-breaking treatments. Both HT and HC treatments significantly breaking bud dormancy,inhibited CAT activity and induced H2O2 accumulation, but showed little effects on POD activity. TDZ showed poor dormancy-breaking efficiency and no significant effects on H2O2 content, CAT activity and POD activity.Inducing H2O2 accumulation through CAT inhibition might be an important part of dormancy-breaking mechanism induced by HT and HC. |
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| Keywords: | dormancy-breaking treatments H2O2 metabolism bud dormancy nectarine |
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