研磨珠辅助提取茶树菇水溶性核苷酸工艺研究

本文以食用真菌茶树菇子实体为原料，采用研磨珠细胞破壁提取法，对其所含的核苷酸类物质进行提取。通过单因素实验，采用响应面分析法优化研磨珠辅助提取工艺参数，并确定最佳提取工艺条件为：研磨时间12min ，液料比为51:1，研磨珠装载量为12.5%，理论核苷酸得率为7.02%。进行二次多元回归拟合，通过回归分析检验多元回归方程模型显著。另外，通过验证试验，实际得率为（6.94±0.07）%，结果与模型符合良好，进一步说明拟合方程的可靠。与传统方法相比，本文采用的研磨珠破壁提取方法，不仅所得核苷酸产品提取率高，并且可大大缩短操作时间，为食用菌及核苷酸的新型生产工艺的开发提供了理论参考。

Extraction process of water-soluble nucleotides from Agrocybe cylindracea by grind bead assisted technology

Water-soluble nucleotide was extracted from fruit body of an edible fungus-Agrocybe cylindracea by the grind bead assisted extraction technology. After the single-factor experiments, the process parameters were optimized through Response Surface Analysis method and the optimum extraction conditions was determined (grind time: 12min, ratio of liquid to solid: 51:1, loading capacity of grind beads: 12.5%). The theoretical yield for nucleotide was 7.02%. After a quadratic multiple regressions fit, a multiple regression equation was achieved and identified significant though regression analysis. Additionally, the actual yield was (6.94±0.07)% by the confirmatory test, which was according with theoretical result and thereby illustrated the fitted multiple regression equation credible. Compared with traditional methods, grind bead assisted extraction method in this article not only produced high yield of nucleotides but greatly shortened process time, which provided an industrial reference for developing new processes for edible fungus and nucleotide.