| 高效液相色谱法测定硫酸小檗碱注射液的含量 |
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| 引用本文： | 李华岑. 高效液相色谱法测定硫酸小檗碱注射液的含量[J]. 中国兽药杂志, 2011, 45(10): 31-33 |
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| 作者姓名： | 李华岑 |
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| 作者单位： | 河南省兽药监察所,郑州,450008 |
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| 摘 要： | 以C18为固定相,0.1%磷酸溶液（用三乙胺调节pH值至3.5±0.1）—乙腈（70∶30）为流动相,229 nm为检测波长,采用高效液相色谱法测定了硫酸小檗碱注射液中硫酸小檗碱的含量。盐酸小檗碱线性范围为0.2～2.5μg,回归方程为A=4 161 950 m＋408 151,相关系数为0.999 9。平均加标回收率为100.8%,RSD为2.2%（n=9）。
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| 关 键 词： | 硫酸小檗碱注射液 硫酸小檗碱 盐酸小檗碱 含量 高效液相色谱法 |
| 收稿时间： | 2011-07-25 |
| 修稿时间： | 2011-08-19 |
Determination of Berberine Sulfate Injection by HPLC |
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| LI Hua-cen. Determination of Berberine Sulfate Injection by HPLC[J]. Chinese Journal of Veterinary Drug, 2011, 45(10): 31-33 |
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| Authors: | LI Hua-cen |
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| Affiliation: | Henan Provincial Supervisory Institute of Veterinary Drug |
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| Abstract: | An HPLC method has been developed to determine the berberine sulfate injection,using C18 column with a mobile of 0.1% phosphoric solution（adjusted pH value to 3.5±0.1 with triethylamine）-acetonitrile（70∶ 30） and wavelength of 229 nm.The linear range of berberine hydrochloride was 0.2~2.5 μg.And the regression equation was A=4161950m＋408151 with the correlation to be 0.9999.The average recovery was 100.8% with RSD of 2.2%（n=9）. |
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| Keywords: | berberine sulfate injection berberine sulfate berberine hydrochloride content HPLC |
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