| 绵羊肌肉IGF-Ⅰ基因表达的发育性变化研究 |
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| 引用本文： | 黄治国,谢庄.绵羊肌肉IGF-Ⅰ基因表达的发育性变化研究[J].农业科学与技术,2009,10(2). |
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| 作者姓名： | 黄治国 谢庄 |
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| 作者单位： | 黄治国,HUANG Zhi-guo(四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000;南京农业大学动物科技学院,江苏南京210095);谢庄,XIE Zhuang(南京农业大学动物科技学院,江苏南京,210095)
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| 基金项目： | 新疆生产建设兵团博士基金 |
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| 摘 要： | Objective] To detect the mRNA of muscle insulin-like growth factorⅠ (IGF-Ⅰ) in the early development of Kazak sheep and Xinjiang fine-wool sheep, so as to provide information for the research about the early growth and development of sheep. Method] With real-time quantitative PCR, the muscle IGF-Ⅰ mRNA level was separately detected in two varieties of sheep at 2, 30, 60, 90 and 120 days old. Then the data was analyzed with SPSS software. Result] The IGF-Ⅰ mRNA in sheep muscle first increased and then decreased with ages, peaking at 30 days old in Kazak sheep and at 60 days old in Xinjiang fine-wool sheep. The IGF-Ⅰ expression level of Kazak sheep had no significant difference with Xinjiang fine-wool sheep at 2 or 90 days old (P>0.05), but was lower than that of the latter with extremely significant difference (P<0.01) from 30 to 60 days old. Conclusion] The male Kazak sheep and Xinjiang fine-wool sheep have similar model of developmental changes of muscular IGF-Ⅰ mRNA, but the expression level is different between these two species.
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| 关 键 词： | IGF-Ⅰ |
Developmental Changes of IGF-Ⅰ mRNA Expression Level in Sheep Muscle |
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| HUANG Zhi-guo,XIE Zhuang.Developmental Changes of IGF-Ⅰ mRNA Expression Level in Sheep Muscle[J].Agricultural Science ＆ Technology,2009,10(2). |
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| Authors: | HUANG Zhi-guo XIE Zhuang |
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| Abstract: | |
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| Keywords: | Sheep Expression |
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