| HPLC测定野葛中葛根素含量的研究 |
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| 引用本文： | 赵喜兰. HPLC测定野葛中葛根素含量的研究[J]. 安徽农业科学, 2010, 38(34): 19298-19298,19309. DOI: 10.3969/j.issn.0517-6611.2010.34.023 |
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| 作者姓名： | 赵喜兰 |
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| 作者单位： | 漯河医学高等专科学校,河南漯河,462002 |
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| 摘 要： | [目的]建立野葛中葛根素含量的测定方法。[方法]采用高效液相色谱法,Diamonsil C18（250.0 mm×4.6 mm,5.0μm）,流动相为甲醇∶水（80∶10,V/V）,紫外检测波长250 nm。[结果]葛根素在0.242～2.420 mg/ml范围内线性关系良好,加样回收率为99.29%。[结论]该法简便、快速、重复性好,可用于测定野葛中葛根素含量。
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| 关 键 词： | 野葛 葛根素 高效液相色谱法 |
Determination of Puerarin in Pueraria lobata (Willd) by HPLC |
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| ZHAO Xi-lan. Determination of Puerarin in Pueraria lobata (Willd) by HPLC[J]. Journal of Anhui Agricultural Sciences, 2010, 38(34): 19298-19298,19309. DOI: 10.3969/j.issn.0517-6611.2010.34.023 |
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| Authors: | ZHAO Xi-lan |
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| Affiliation: | ZHAO Xi-lan(Luohe Medical College,Luohe,Henan 462002) |
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| Abstract: | [Objective] The determination of puerarin content of Pueraria lobata(Willd) was established.[Method]HPLC was adopted.The analysis was performed on a Diamonsil C18 column.The mobile phase was methanol-water(80∶10,V/V).The detection wavelength was 250 nm.[Result] The linear range of 0.242-2.42 mg/ml was good.The average recovery was 99.29%.[Conclusion] The method is simple and rapid,and it could be used for the quantitative analysis of puerarin in Pueraria lobata(Willd). |
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| Keywords: | Pueraria lobata(Willd) Puerarin HPLC |
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