Faecal carriage of extended spectrum beta-lactamase (ESBL) and New Delhi metallo beta-lactamase(NDM) producing Escherichia coli between piglets and pig farmworkers |
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| Affiliation: | 1. Department of Microbiology, Virgen de las Nieves University Hospital-ibs, Granada, Spain;2. Department of Microbiology, Virgen Macarena University Hospital, Seville, Spain;3. Bacteriology-Hygiene unit, Assistance Publique/Hôpitaux de Paris (AP-PH), Bicêtre Hospital, Le Kremlin-Bicêtre, France;4. Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bicêtre, France;5. EA7361 “Structure, dynamic, function and expression of broad spectrum β-lactamases”, Université Paris-Sud, Université Paris-Saclay, LabEx LERMIT, Faculty of Medicine, Le Kremlin-Bicêtre, France;6. Joint Research Unit EERA «Evolution and Ecology of Resistance to Antibiotics», Institut Pasteur - APHP - Université Paris Sud, Paris, France;7. Department of Preventive Medicine, Virgen de las Nieves University Hospital, Granada, Spain;1. Department of Clinical Microbiology, First Affiliated Hospital of Nanchang University, Nanchang University, Yong Wai Zheng Jie No. 17, Nanchang 330006, China;2. Department of Infection Disease, First Affiliated Hospital of Nanchang University, Nanchang University, Yong Wai Zheng Jie No. 17, Nanchang 330006, China;3. Institute of Immunotherapy and College of Basic Medicine, Jiangxi Academy of Medical Sciences, Nanchang University, Ba Yi Road No. 603, Nanchang 330006, China |
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| Abstract: | A cross-sectional study on five organized pig farms was conducted to assess the faecal carriage of ESBL and blaNDM carbapenemase-producing E. coli in piglets and pig farmworkers. Faecal samples from piglets (n = 155) and pig farmworkers (n = 21) were processed for isolation and characterization of E. coli. A total of 124 E. coli isolates from piglets and 21 E. coli isolates pig farmworkers were recovered and screening for ESBL production showed that 44.4 % (55/124) of the isolates from piglets and 42.9 % (9/21) of the isolates from farmworkers were ESBL positive. The ESBL positive isolates from piglets and farmworkers harbored blaCTX-M and also co-harbored other beta-lactams, sulphonamide, quinolone and tetracycline resistance genes. Diarrhoeic (50%, 49/98) and crossbred piglets (52.7%, 39/74) harbored a significantly higher number of ESBL producing isolates than non-diarrhoeic (23.1 %, 6/26) and purebred piglets (32%, 16/50) (p < 0.05). Piglets and pig farmworkers harbored nine and two carbapenem-resistant isolates, respectively. Interestingly, two isolates from piglets and one isolate from farmworkers harbored the blaNDM gene. The blaNDM positive E. coli isolated from piglets and farmworkers of the same farm revealed similar antibacterial resistance patterns, resistant genes, sequence (ST-167) and plasmid type (IncX3). In India, carbapenems are not used in food animal treatment, hence carbapenem resistant E. coli in piglets possibly originated from the human contact or common environment and is of public health importance. |
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| Keywords: | carbapenem resistance plasmid piglets farmworkers India |
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