无花果炭疽病菌的生物学特性及8种杀菌剂对其抑制作用

 通过十字交叉法和血球计数板计数法测定无花果炭疽病菌的生物学特性及其对8种杀菌剂的敏感性,结果表明,菌丝体生长、产孢的最适温度都为25～30℃,最适生长pH值为5~6。同时明确了不同碳、氮源对无花果炭疽病菌菌落生长及其产孢的影响。氟硅唑和戊唑醇抑制毒力最高,EC₅₀分别为0.025 7 μg·mL^-1和0.183 μg·mL^-1,分别为醚菌酯的1 395.55和195.99倍;其次为苯醚甲环唑和异菌脲,EC₅₀分别为0.187 9 μg·mL^-1和0.434 9 μg·mL^-1;醚菌酯毒力较小,不适合用于防治无花果炭疽病。

Biological characteristics of Colletotrichum gloeosporioides and inhibitory effects of eight fungicides

By using the crossing and haemocytometer method, the growth, spore production and other biological characteristics of Colletotrichum gloeosporioides were determined. In order to identify effective fungicides to control Colletotrichum gloeosporioides, toxicity of eight fungicides was tested by mycelial growth rate method. The results showed that the optimum temperature was 25-30℃ and the optimum pH was 5-6. The effective concentrations of the eight fungicides were obviously different. The inhibitory activities of flusilazole and tebuconazole were the highest with EC₅₀ of 0.025 7 and 0.183 μg·mL^-1, which were 1 395.55 and 195.99 times of Kresoxim-methyl, respectively. The inhibitory activities of difenoconazole and iprodione were the second with EC₅₀ of 0.187 9 and 0.434 9 μg·mL^-1. The inhibitory activities of kresoxim-methyl were the lowest.