| 马MxA基因第13外显子的多态性研究(英文) |
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| 引用本文： | 萨仁高娃,菊林花. 马MxA基因第13外显子的多态性研究(英文)[J]. 农业科学与技术, 2008, 9(6): 25-26 |
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| 作者姓名： | 萨仁高娃 菊林花 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特,010018 |
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| 基金项目： | 内蒙古自治区自然科学基金 |
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| 摘 要： | [Objective] To investigate the polymorphism of the thirteenth exon of MxA gene in 4 species of horse. [Method] The thirteenth exon of MxA gene fragments were amplified from genomic DNA of Sanhe horse, Xinihe horse, Wushen horse and Baerhu horse with the primers designed according to the MxA sequence announced in GenBank; the polymorphism of MxA gene was detected by PCR-SSCP and the products were sequenced. [Result] The polymorphism of the thirteenth exon of MxA gene appeared only in Wushen horse, the 2 081 nt of which mutated from guanine (G) to adenine (A) and the corresponding amino acid of which changed from glutamate (Glu) to alanine (Ala). [Conclusion] The study provided a basis for exploring the antiviral effect of MxA protein.
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| 关 键 词： | Horse MxA gene Polymorphism PCR-SSCP |
Polymorphism Analysis of the Thirteenth Exon of Equine MxA Gene |
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| Sarengaowa,JU Lin-hua. Polymorphism Analysis of the Thirteenth Exon of Equine MxA Gene[J]. Agricultural Science ＆ Technology, 2008, 9(6): 25-26 |
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| Authors: | Sarengaowa JU Lin-hua |
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| Affiliation: | College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018 |
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| Abstract: | [Objective] To investigate the polymorphism of the thirteenth exon of MxA gene in 4 species of horse. [Method] The thirteenth exon of MxA gene fragments were amplified from genomic DNA of Sanhe horse, Xinihe horse, Wushen horse and Baerhu horse with the primers designed according to the MxA sequence announced in GenBank; the polymorphism of MxA gene was detected by PCR-SSCP and the products were sequenced. [Result] The polymorphism of the thirteenth exon of MxA gene appeared only in Wushen horse, the 2 081 nt of which mutated from guanine (G) to adenine (A) and the corresponding amino acid of which changed from glutamate (Glu) to alanine (Ala). [Conclusion] The study provided a basis for exploring the antiviral effect of MxA protein. |
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| Keywords: | Horse MxA gene Polymorphism PCR-SSCP |
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