| 一种快速分离小麦条锈菌转GUS基因转化体的方法 |
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| 引用本文： | 卢丽丽,张亮,严引娣,王美南,王阳,井金学. 一种快速分离小麦条锈菌转GUS基因转化体的方法[J]. 西北农业学报, 2009, 18(1): 170-172,183 |
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| 作者姓名： | 卢丽丽 张亮 严引娣 王美南 王阳 井金学 |
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| 作者单位： | 西北农林科技大学,植物保护学院,陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金，陕西省攻关项目，陕西省自然科学基金青年项目，教育部长江学者和创新团队发展计划 |
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| 摘 要： | 为了获得更多整合有外源GUS报告基因并且稳定表达的小麦条锈菌毒性突变菌株.探索小麦条锈菌夏孢子转GUS基因遗传转化体的分离方法.以从菌种筛选品种上分离转化体法为主、从繁殖品种辉县红上分离转化体法为辅可有效提高转化体的检出率;建立了利用透明胶带粘取夏孢子并通过染色分离小麦条锈菌转GUS基因转化体的新方法.试验证明,透明胶带粘取夏孢子染色法是一种快速有效分离小麦条锈菌转GUS基因转化体的方法.
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| 关 键 词： | 小麦条锈菌 GUS转化体 分离方法 |
| 收稿时间： | 2008-05-04 |
| 修稿时间： | 2008-09-28 |
A Rapid Method to Separate the Transformants with GUS Gene in Puccinia striiformis |
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| LU Lili,ZHANG Liang,YAN Yindi,WANG Meinan,WANG Yang and JING Jinxue. A Rapid Method to Separate the Transformants with GUS Gene in Puccinia striiformis[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(1): 170-172,183 |
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| Authors: | LU Lili ZHANG Liang YAN Yindi WANG Meinan WANG Yang JING Jinxue |
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| Affiliation: | College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | To obtain more virulent mutant stains with GUS gene in Puccinia striiformis.The separation method of the transformants with GUS Gene in wheat stripe rust was studied.The result showed that to separate transformants from screening cultivars while at the same time separating transformants from breeding cultivar Huixianhong as an assistant way can increase the detectable rate efficiently. A new way to obtain the transformants with GUS gene through pasting and dyeing of uredio spores of P.striiformis by transparent tape was established.The new method is an effective way to separate the transformants with GUS gene. |
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| Keywords: | Puccinia striiformis GUS-transformants Separation method |
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