裸小鼠人结肠癌术后转移模型的建立

将人结肠腺癌细胞株HCT-116接种于裸小鼠右侧背部近腋部皮下,得到皮下移植瘤,肿瘤在体内适应后用28只裸小鼠采用组织块法建立皮下移植瘤模型。4周后随机挑选15只裸小鼠作为实验组切除肿瘤组织,建立裸小鼠人结肠癌术后转移模型,其余13只裸小鼠作为对照组,观察动物生存情况及肿瘤远处转移情况。对照组裸小鼠9周时出现恶病质,解剖检查肺转移率为23%(3/13);实验组裸小鼠术部无肿瘤复发,17周后肺转移率达到100%(15/15),淋巴结转移率100%(15/15),脾脏转移率33.3%(5/15)。本模型模拟了临床肿瘤根除术后发生远处转移的过程,可为研究结肠癌转移机制和术后抗转移治疗提供理想的动物模型。

Establishment of Human Colorectal Cancer Postoperative Metastasis Model in Nude Mice

To establish a human colorectal cancer postoperative metastasis model in nude mice,so as to provide reliable animal model to the study and treatment of colorectal metastasis.Tumor cell line HCT-116 of human colorectal adenocarcinoma was inoculated subcutaneously into nude mice to develop an implantation tumor,which was then used to establish the colorectal cancer metastasis model.Histological intact tumor tissue was then harvested and implanted to the subcutaneous of 28 nude mice.Four weeks later,15 of them were randomly selected as experimental group and had tumor resection.The information of mice survival and tumor metastasis rates were collected.Mice in the control group died after 9 weeks with 23%(3/13)of them had lung metastasis.While in the experimental group,the metastasis rates in lung,lymph node,and spleen were 100%(15/15),100%(15/15)and 33.3%(5/15)respectively at the end of 17 weeks when mice were sacrificed.