| Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase （SPS）Activity in Two Tomato Species |
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| 引用本文： | WANG Li,CUI Na,ZHAO Xiao-cui,FAN Hai-yan,LI Tian-lai. Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase （SPS）Activity in Two Tomato Species[J]. 农业科学学报, 2014, 13(2): 358-364. DOI: 10.1016/S2095-3119(13)60373-1 |
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| 作者姓名： | WANG Li CUI Na ZHAO Xiao-cui FAN Hai-yan LI Tian-lai |
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| 作者单位： | [1]Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, P.R. China [2]Key Laboratory of Protected Horticulture, Ministry of Education/College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China |
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| 基金项目： | This study was supported by the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2011BAD12B03). |
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| 摘 要： | To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase （SPS） activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography （HPLC）. The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase （AI） and neutral invertase （NI） in Micro-Tom （Solanum lycopersicum） fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period.
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| 关 键 词： | 14-3-3蛋白 蔗糖磷酸合成酶 碳水化合物代谢 SPS活性 活性测定 番茄品种 积累 RT-PCR检测 |
| 收稿时间： | 2012-11-10 |
Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species |
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| WANG Li,CUI Na,ZHAO Xiao-cui,FAN Hai-yan,LI Tian-lai. Accumulation of Carbohydrate and Regulation of 14-3-3 Protein on Sucrose Phosphate Synthase (SPS) Activity in Two Tomato Species[J]. Journal of Integrative Agriculture, 2014, 13(2): 358-364. DOI: 10.1016/S2095-3119(13)60373-1 |
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| Authors: | WANG Li CUI Na ZHAO Xiao-cui FAN Hai-yan LI Tian-lai |
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| Affiliation: | 1.Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110866, P.R.China2.Key Laboratory of Protected Horticulture, Ministry of Education/College of Horticulture, Shenyang Agricultural University, Shenyang 110866,P.R.China |
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| Abstract: | To explore the differences of carbohydrate metabolism in two tomato species and discuss the possible regulation of 14-3-3 proteins on the sucrose phosphate synthase (SPS) activity, we determined the contents of soluble sugar and starch through high performance liquid chromatography (HPLC). The activities of sugar-metabolizing enzymes were assayed in desalted extract, and the relative expression levels of related genes in sugar metabolism were determined though real-time RT-PCR. The results indicated that glucose and fructose were mainly accumulated during the maturation of the fruit because of the high acid invertase (AI) and neutral invertase (NI) in Micro-Tom (Solanum lycopersicum) fruit, while in Solanum chmielewskii fruit, SPS which went along with the change of sucrose content led to the rapid sucrose increase during the fruit ripening. TFT1 and TFT10, belonging to 14-3-3 protein in tomato, were likely to down-regulated SPS activity during young and intumescence period. |
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| Keywords: | Micro-Tom Solanum chmielewskii carbohydrate accumulation 14-3-3 proteins fruit development |
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