Anti-obesity activity of diglyceride containing conjugated linoleic acid in C57BL/6J ob/ob mice |
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Authors: | Jin-Joo Hue Ki Nam Lee Jae-Hwang Jeong Sang-Hwa Lee Young Ho Lee Seong-woon Jeong Sang Yoon Nam Young Won Yun Beom Jun Lee |
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Affiliation: | 1College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.;2Department of Biotechnology and Biomedicine, Chungbuk Province College, Okcheon 373-807, Korea.;3Department of Food and Nutrition, Seowon University, Cheongju 361-742, Korea.;4Ilshinwells, Cheongwon 363-890, Korea. |
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Abstract: | This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DG-CLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism. |
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Keywords: | conjugated linoleic acid C57BL/6J ob/ob mouse diglyceride obesity PPAR-γ |
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