| 普通PCR与TD-PCR扩增叶尔羌高原鳅抗菌肽Hepcidin基因的比较 |
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| 引用本文： | 刘书东,王娟娟,陈根元,李莲瑞.普通PCR与TD-PCR扩增叶尔羌高原鳅抗菌肽Hepcidin基因的比较[J].塔里木农垦大学学报,2011(4):40-45. |
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| 作者姓名： | 刘书东 王娟娟 陈根元 李莲瑞 |
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| 作者单位： | 塔里木大学生命科学学院;塔里木畜牧科技兵团重点实验室 |
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| 基金项目： | 新疆生产建设兵团博士资金(2009JCl8) |
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| 摘 要： | 为了比较普通PCR和降落PCR( TD - PCR)扩增Hepcidin基因的效果,通过Trizol法提取叶尔羌高原鳅肝胰脏总RNA并合成cDNA,分别利用普通PCR和降落PCR扩增Hepcidin基因片段.结果显示,降落PCR能有效扩增叶尔羌高原鳅Hepcidin.测序结果显示:叶尔羌高原鳅抗菌肽Hepcidin基因...
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| 关 键 词： | 降落PCR 叶尔羌高原鳅 Hepcidin基因 |
Comparison of Ordinary PCR and Landing PCR Amplification ofHepcidin gene from Triplophysa(Hedinichthys) yarkandensis(Day) |
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| Liu Shudong,Wang Juanjuan,Chen Genyuan,Li Lianrui.Comparison of Ordinary PCR and Landing PCR Amplification ofHepcidin gene from Triplophysa(Hedinichthys) yarkandensis(Day)[J].Journal of Tarim University of Agricultural Reclamation,2011(4):40-45. |
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| Authors: | Liu Shudong Wang Juanjuan Chen Genyuan Li Lianrui |
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| Institution: | 1 College of Life Science,Tarim University,Alar,Xinjiang 843300)(2 Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production and Construction Group,Alar,Xinjiang 843300) |
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| Abstract: | In order to compare the effect of Hepcidin gene amplification by odinary PCR and the landing PCR(TD-PCR),the cDNA from total RNA synthesis of Triplophysa yarkandensis’s hepatopancreas is respectively used to amplify Hepcidin gene fragment using common PCR and landing PCR.The results showed that the landing PCRcan effectively amplified Hepcidin gene.The sequencing results showed that the Hepcidin gene homology of Triplophysa yarkandensis and the ordinary fish was up to 90%,while the ordinary PCR did not enlarge apparent purpose strip.The results of this study show that in the same reaction system,landing PCR without affecting the specificity,can increase amplification efficiency,and can be used for PCR amplification of ordinary hard-amplified gene fragments. |
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| Keywords: | TD-PCR Triplophysa(Hedinichthys) yarkandensis(Day) Hepcidin gene |
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