| 兔IL-6基因真核表达载体的构建及其对pcDNA-VP60DNA疫苗佐剂效应研究 |
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| 引用本文： | 张夏兰. 兔IL-6基因真核表达载体的构建及其对pcDNA-VP60DNA疫苗佐剂效应研究[J]. 中国动物传染病学报, 2012, 20(5): 12-15 |
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| 作者姓名： | 张夏兰 |
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| 作者单位： | 重庆市巴南区动物疫病预防控制中心,重庆,401320 |
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| 摘 要： | 为研究兔白介素6（interleukin6，IL-6）真核表达质粒（pcDNA-IL-6）对核酸疫苗免疫效果的影响，本文构建了真核表达质粒pcDNA-IL-6，并将其与核酸疫苗pcDNA-VP60联合免疫家兔，以pcDNA-VP60和质粒载体pcDNA3．1（＋）作对照，用血凝抑制试验检测试验兔体内特异性抗体水平。结果表明：真核重组质粒pcDNA-IL-6对重组质粒pcDNA-VP60均具有免疫增强作用，从免疫后7d到70d，pcDNA-VP60／pcDNA-IL6联合免疫组抗体水平均高于pcDNA-VP60免疫组，差异具有显著统计学意义。
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| 关 键 词： | 兔病毒性出血症 DNA疫苗 佐剂 白细胞介素6 |
ENHANCEMENT OF EUKARYOTIC EXPRESSION PLASMID pcDNA-IL-6 ON IMMUNE RESPONSE OF DNA VACCINE pcDNA-VP60 AGAINST RABBIT HEMORRHAGIC DISEASE VIRUS |
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| ZHANG Xia-lan. ENHANCEMENT OF EUKARYOTIC EXPRESSION PLASMID pcDNA-IL-6 ON IMMUNE RESPONSE OF DNA VACCINE pcDNA-VP60 AGAINST RABBIT HEMORRHAGIC DISEASE VIRUS[J]. Chinese Journal of Animal Infectious Diseases, 2012, 20(5): 12-15 |
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| Authors: | ZHANG Xia-lan |
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| Affiliation: | ZHANG Xia-lan(Chongqing Banan Animal Disease Control Center,Chongqing 401320,China) |
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| Abstract: | To investigate the effect of eukaryotic expression plasmid pcDNA-IL-6 on the immune response of DNA vaccine against Rabbit hemorrhagic disease virus(RHDV),eukaryotic expression plasmid pcDNA-VP60 expressing RHDV VP60 protein were constructed and inoculated into rabbits alone or simultaneously with pcDNA-IL-6.Hemagglutination inhibition(HI) test was used to detect RHDV antibody level.The results indicated that pcDNA-IL-6 enhanced the immune response of pcDNA-VP60.The HI antibody level in pcDNA-VP60+pcDNA-IL6 group was significantly higher than pcDNA-VP60 alone group(P<0.01) as detected from 7 to 70 days post inoculation,the antibody level of group pcDNA-VP60+pcDNA-IL6 was significantly higher than group pcDNA-VP60 and group pcDNA3.1(P<0.05 or P<0.01) |
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| Keywords: | Rabbit hemorrhagic disease (RHD) DNA vaccine adjuvant interleukin 6 (IL-6) |
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