Septin-mediated plant cell invasion by the rice blast fungus, Magnaporthe oryzae |
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Authors: | Dagdas Yasin F Yoshino Kae Dagdas Gulay Ryder Lauren S Bielska Ewa Steinberg Gero Talbot Nicholas J |
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Affiliation: | School of Biosciences, University of Exeter, Exeter, UK. |
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Abstract: | To cause rice blast disease, the fungus Magnaporthe oryzae develops a pressurized dome-shaped cell called an appressorium, which physically ruptures the leaf cuticle to gain entry to plant tissue. Here, we report that a toroidal F-actin network assembles in the appressorium by means of four septin guanosine triphosphatases, which polymerize into a dynamic, hetero-oligomeric ring. Septins scaffold F-actin, via the ezrin-radixin-moesin protein Tea1, and phosphatidylinositide interactions at the appressorium plasma membrane. The septin ring assembles in a Cdc42- and Chm1-dependent manner and forms a diffusion barrier to localize the inverse-bin-amphiphysin-RVS-domain protein Rvs167 and the Wiskott-Aldrich syndrome protein Las17 at the point of penetration. Septins thereby provide the cortical rigidity and membrane curvature necessary for protrusion of a rigid penetration peg to breach the leaf surface. |
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