| 用大肠杆菌K99和F41菌毛抗原检测牛血清抗体的琼脂扩散试验的研究 |
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| 引用本文： | 崔玉东 杜学昆. 用大肠杆菌K99和F41菌毛抗原检测牛血清抗体的琼脂扩散试验的研究[J]. 黑龙江畜牧兽医, 1998, 0(4): 3-5 |
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| 作者姓名： | 崔玉东 杜学昆 |
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| 作者单位： | 黑龙江八一农垦大学动科院，新华农场兽医院，双城市兽医卫生防疫站 |
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| 摘 要： | 用含２ｍｏｌ／Ｌ尿素的磷酸盐缓冲液以热处理方法提取的Ｋ９９和Ｆ４１菌毛作为抗原，对琼脂扩散试验方法进行研究。结果表明本试验方法简便，特异性强，敏感性高，可用于血清抗体检测；所制备的抗原稳定、重复性好。
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| 关 键 词： | 大肠杆菌 K_（99）和F_（41）菌毛抗原 琼脂扩散试验 检测抗体 |
Studies on Agar Gel Diffusion Test Wity K 99 and F 41 Pili Antigens Enterotoxigenic E.coli for Detection Antibodies in Bovine Sera |
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| Cui Yudong Du XueKun Zhang Shouhong et al. Studies on Agar Gel Diffusion Test Wity K 99 and F 41 Pili Antigens Enterotoxigenic E.coli for Detection Antibodies in Bovine Sera[J]. Heilongjiang Animal Science And veterinary Medicine, 1998, 0(4): 3-5 |
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| Authors: | Cui Yudong Du XueKun Zhang Shouhong et al |
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| Abstract: | Agar gel diffusion test was studied with K 99 and F 41 piliantigens extracted by heat treatment in PBS containing 2 M urea.This test was simple,sensitive,high specific and suitable for detecting K 99 and F 41 antibodies in bovine sera.The prepared antigens were stable and repeatable. |
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| Keywords: | Enterotoxigenic Escherichia coli K 99 and F 41 pili antigens Agar gel diffusion test Detecting antibody |
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