转基因植物中Bt Cry2Ab蛋白的抗原表位特征预测 |
| |
引用本文: | 高洁荣,何颖,邹泽红,陶爱林,艾云灿. 转基因植物中Bt Cry2Ab蛋白的抗原表位特征预测[J]. 安徽农业科学, 2012, 40(25): 12400-12402,12586 |
| |
作者姓名: | 高洁荣 何颖 邹泽红 陶爱林 艾云灿 |
| |
作者单位: | 中山大学生命科学院,有害生物控制与资源利用国家重点实验室,广东广州510275;广州医学院第二附属医院,呼吸疾病国家重点实验室变态反应研究室,过敏反应与临床免疫重点实验室,广东广州510260;广州医学院第二附属医院,呼吸疾病国家重点实验室变态反应研究室,过敏反应与临床免疫重点实验室,广东广州510260;中山大学生命科学院,有害生物控制与资源利用国家重点实验室,广东广州510275 |
| |
基金项目: | 国家科技重大专项重大课题(2011ZX08011-005)及重点课题(2009ZX08011-004B);国家自然基金(30771240);呼吸疾病国家重点实验室(广州医学院)课题研究基金 |
| |
摘 要: | [目的]通过生物信息学方法了解转基因作物中Bt Cry2Ab蛋白的二级结构、三级结构、B细胞抗原表位及T细胞抗原表位等结构特征,为今后的抗体设计奠定基础。[方法]以在NCBI数据库中获得Cry2Ab蛋白序列为材料,应用DNAStar中的多种方法预测其B细胞表位,并通过在线预测软件NetMHCII 2.2分析Cry2Ab蛋白与MHC-II类分子的结合能力,从而预测其T细胞抗原表位。[结果]对Cry2Ab蛋白B细胞抗原表位的预测表明,Cry2Ab蛋白的第208~215区域是潜在的B细胞抗原表位;对Cry2Ab蛋白与MHC-II类分子结合能力的分析表明,Cry2Ab蛋白的第177~185区域、299~307区域和255~263区域是潜在的T细胞抗原表位,暗示含有HLA-DRB10101和HLA-DRB10701等位基因的人群对Cry2Ab蛋白更敏感。[结论]该研究有助于深入了解Cry2Ab蛋白的生物学特征,为完善转基因食品过敏原性的评估方法提供新线索。
|
关 键 词: | 转基因植物 过敏原性 Cry2Ab蛋白 B细胞表位 T细胞表位 |
Prediction on Antigenic Epitope Characteristics of Bt Cry2Ab Protein in Transgenic Crops |
| |
Affiliation: | GAO Jie-rong et al(State Key Laboratory of Biocontrol,School of Life Sciences,Sun Yat-Sen University,Guangzhou,Guangdong 510275) |
| |
Abstract: | [Objective] This study aimed to predict the structural characteristics of Bt Cry2Ab protein in transgenic crops with bioinformatic analysis to provide the theoretical clues for design of antibody Cry2Ab.[Method] The amino acid sequence of Cry2Ab protein was searched from NCBI database.The B cell epitopes were predicted with DNAStar.The binding affinity between Cry2Ab protein and MHC-II molecules was analyzed with NetMHCII 2.2 Server to predict the T cell epitopes.[Result] Prediction result suggested the potential B cell epitope of Cry2Ab locating in the region of 208-215.Analysis of the binding affinity between Cry2Ab and MHC-II molecules suggested the regions of 177-185,299-307 and 255-263 were the potential T cell epitopes.Human with HLA-DRB10101 alleles and HLA-DRB10701 alleles were more sensitive to Cry2Ab protein.[Conclusion] This study facilitates to understand the structural characteristics of Cry2Ab protein and provides a new clue to improve the assessment method for potential allergenicity of genetically modified food. |
| |
Keywords: | Transgenic crops Allergenicity Cry2Ab B cell epitopes T cell epitopes |
本文献已被 CNKI 万方数据 等数据库收录! |
|