柴胡提取物中总皂苷测定方法研究 |
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引用本文: | 陈淑,魏亚琴,索志荣. 柴胡提取物中总皂苷测定方法研究[J]. 安徽农业科学, 2012, 40(24): 11998-11999,12002 |
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作者姓名: | 陈淑 魏亚琴 索志荣 |
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作者单位: | 西南科技大学分析测试中心,四川绵阳,621010;西南科技大学分析测试中心,四川绵阳,621010;西南科技大学分析测试中心,四川绵阳,621010 |
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基金项目: | 西南科技大学-四川德培源中药科技开发有限公司校企联合基金目(11zh0093) |
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摘 要: | [目的]考察柴胡提取物中总皂苷的最佳测定方法。[方法]以总皂苷吸光度为评价指标,采用单因素试验优选柴胡总皂苷的测定方法。[结果]总皂苷的最佳测定方法为:供试品溶液0.2 ml,加入0.4%对二甲氨基苯甲醛乙醇溶液0.1 ml在70℃反应10 min,放冷后至室温后,加入4 ml磷酸在50℃下反应20 min。[结论]该方法稳定可行,适用于柴胡总皂苷的含量测定。
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关 键 词: | 紫外分光光度法 柴胡(RADIX BUPLEURI) 总皂苷 测定方法 |
Research on the Determination Method of Total Saponin from RADIX BUPLEURI Extracts |
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Affiliation: | CHEN Shu et al(Center of Instrumental Analysis,Southwest University of Science and Technology,Mianyang,Sichuan 621010) |
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Abstract: | [Objective] To set up an optimal method for the determination of total saponins from RADIX BUPLEURI extracts.[Method] The determination method was optimized by single factor test according to the absorbance of total saponin.[Result] The best determination method of total saponin was: 0.2 ml test solution was added with 0.1 ml 0.4% p-dimethylaminobenzaldehyde ethanol solution under 70 ℃ thermostatic waterbath for 10 minutes,after cooling off,4 ml phosphoric acid was added under 50 ℃ thermostatic waterbath for 20 minutes.[Conclusion] The method is table and practical,and it is applicable to the determination of total saponin from RADXI BUPLEURI extracts. |
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Keywords: | UV-Vis spectrophotometry RADIX BUPLEURI Total saikosaponins Determination method |
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