鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定 |
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引用本文: | 龙子文,周杰,王春台,徐鑫.鄂西地区稻瘟病菌生理小种文库的构建及初步鉴定[J].安徽农业科学,2012,40(24):12065-12067. |
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作者姓名: | 龙子文 周杰 王春台 徐鑫 |
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作者单位: | 中南民族大学生命科学学院,生物技术国家民委重点实验室,湖北武汉430074;中南民族大学生命科学学院,生物技术国家民委重点实验室,湖北武汉430074;中南民族大学生命科学学院,生物技术国家民委重点实验室,湖北武汉430074;中南民族大学生命科学学院,生物技术国家民委重点实验室,湖北武汉430074 |
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基金项目: | 湖北省自然科学基金创新群体“武陵山区稻瘟病菌收集分类与抗病育种研究”(2011CDA125) |
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摘 要: | 目的]构建鄂西地区的稻瘟病菌文库,并对其进行初步的鉴定分析。方法]从鄂西地区7个水稻主产区不同的水稻品种上收集稻瘟病感病稻秆,进行分离、纯化和菌落形态的初步鉴定分析,并利用RAPD和REMAP分子标记对该文库进行聚类分析。结果]建立了含有52种菌株的稻瘟病菌生理小种文库;聚类分析结果表明,鄂西地区的稻瘟病菌群体结构呈现多样性和复杂性,遗传谱系与菌落形态之间并不存在简单的一一对应关系。结论]该研究为预测鄂西地区病原菌群体的变化趋势以及水稻抗病品种的选育,奠定了基础。
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关 键 词: | 稻瘟病菌 生理小种文库 鄂西地区 构建 鉴定 |
Construction and Initial Identification of Physiological Race Library of Magnaporthe oryzea in Western Hubei Province |
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Institution: | LONG Zi-wen et al(Key Laboratory of State Ethnic Affairs Commission for Biological Technology,College of Life Science,South-Central University for Nationalities,Wuhan,Hubei 430074) |
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Abstract: | Objective] The aim was to construct and initially identify the physiological race library of Magnaporthe oryzea in western Hubei Province.Method] M.oryzea infected rice straws were collected from 7 rice main production regions of western Hubei Province.A physiological race library was constructed by single spore isolation,purification and identification of colony morphology.Cluster analysis of this library was conducted by RAPD and REMAP.Result] The results showed that a physiological race library of 52 isolates was constructed.Cluster analysis showed that population structure of M.oryzea demonstrated diversity and complexity,and there was no corresponding relationship between genetic lineages and colony morphology.Conclusion] The study provides a basis for predicting the trend of changes in M.oryzea population and breeding new resistant varieties in western Hubei Province. |
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Keywords: | Magnaporthe oryzea Physiological race library Western Hubei Province Construction Identification |
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