高效液相色谱法测定忍冬藤中木犀草素的含量 |
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引用本文: | 王怀生,赵雪娇,孙玉滨.高效液相色谱法测定忍冬藤中木犀草素的含量[J].安徽农业科学,2012,40(26):12855-12856. |
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作者姓名: | 王怀生 赵雪娇 孙玉滨 |
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作者单位: | 吉林天药药物研发有限公司,吉林长春,130012;吉林天药药物研发有限公司,吉林长春,130012;吉林天药药物研发有限公司,吉林长春,130012 |
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摘 要: | 目的]测定忍冬藤中木犀草素的含量。方法]采用高效液相色谱法测定,色谱柱为Agilent ZORBAX SB-C18(4.6 mm×250 mm,5μm);流动相为无水甲醇-浓度0.3%磷酸水溶液(47∶53,V/V);流速为1 ml/min;柱温为30℃;检测波长为为350 nm。结果]木犀草素的线性范围为0.5~2.5μg/ml,相关系数R=0.999 6,平均回收率为100.71%,RSD为1.9%;3批药材中木犀草素的平均含量为24.05μg/g。结论]该方法简单、快速、可靠,适用于忍冬藤药材的质量控制。
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关 键 词: | 忍冬藤 木犀草素 高效液相色谱 |
Determination of Luteolin Content in CAULIS LONICERAE by HPLC |
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Institution: | WANG Huai-sheng et al(Jilin Natural Pharmatech Co.,Ltd.,Changchun,Jilin 130012) |
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Abstract: | Objective] To determine the luteolin content in CAULIS LONICERAE.Method] The HPLC method was performed on an Agilent ZORBAX SB-C18(4.6 mm×250 mm,5 μm)using an isocratic elution with methanol-water containing 0.3% phosphoric acid(47∶53,V/V) as the mobile phase at a flow rate of 1 ml/min,the column temperature was 30 ℃ and the detection wavelength was set at 350 nm.Result] The linear rangs and regression equation of luteolin was 0.5-2.5 μg/ml with R=0.999 6.The average recovery was 100.71%,RSD=1.9%.The average luteolin content in the three batches of medicine was 24.05 μg/g.Conclusion] This method is simple,rapid and reliable for the quality control of CAULIS LONICERAE. |
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Keywords: | CAULIS LONICERAE Luteolin HPLC |
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