| 四川安岳柑橘衰退病病原鉴定及序列分析 |
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| 引用本文： | 罗雪,杨云飞,阮涛,王亮.四川安岳柑橘衰退病病原鉴定及序列分析[J].安徽农业科学,2012,40(25):12498-12500. |
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| 作者姓名： | 罗雪 杨云飞 阮涛 王亮 |
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| 作者单位： | 贵州省金沙县林业局林政资源管理站,贵州金沙,551800;西南大学植物保护学院,重庆,400716;四川省安岳县柠檬产业局,四川安岳,642350 |
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| 摘 要： | 目的]探明引起四川安岳柑橘衰退病的病原。方法]利用RT-PCR、克隆、测序等技术对四川安岳地区的柑橘类果树进行柑橘衰退病毒(CTV)检测,并对其外壳蛋白(CP)全序列进行了分析。结果]81个疑似CTV侵染的样品中共有18个检测呈阳性,检出率为22.2%,表明安岳地区柑橘类果树衰退病由CTV引起。基于CP全序列同源性比较及进化分析表明,危害该地区柑橘类果树的CTV分属5个组群,亲缘关系较复杂。结论]为安岳地区CTV的防控与防治提供了理论依据。
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| 关 键 词： | 柑橘 柑橘衰退病毒 病原鉴定 序列分析 四川安岳 |
Molecular Identification and Sequence Analysis of the Pathogen Causing Citrus Tristeza Disease in Anyue County of Sichuan Province |
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| Institution: | LUO Xue et al(Management Station of Forest Resource,Jinsha Forestry Bureau,Jinsha,Guizhou 551800) |
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| Abstract: | Objective] The aim was to identify the pathogen to cause citrus tristeza disease in Anyue County of Sichuan Province.Method] The complete nucleotide sequence of citrus tristeza virus(CTV) coat protein(CP) was determined by using RT-PCR,cloning and sequencing techniques.Result] Eighteen CTV isolates(22.2%) were collected in a total of 81 field citrus samples in Anyue,Sichuan province.Sequence comparison and phylogenetic tree of 18 CTV isolates and other CTV isolates indicated that citrus tristeza disease in Anyue was caused by CTV.These diseases belonged to five groups,and the genetic relationship between CTV was complicated.Conclusion] The research provides theoretical basis for the prevention and control of CTV in Anyue area. |
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| Keywords: | Citrus Citrus tristeza virus(CTV) Pathogen identification Sequence analysis Anyue County of Sichuan Province |
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