| 花生壳中白藜芦醇的超声提取及HPLC检测 |
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| 引用本文： | 刘常金,杨婷,杨家森,赵.花生壳中白藜芦醇的超声提取及HPLC检测[J].安徽农业科学,2009,37(28). |
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| 作者姓名： | 刘常金 杨婷 杨家森 赵 |
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| 作者单位： | 1. 天津科技大学食品工程与生物技术学院,天津,300457
2. 中国药科大学生命科学与技术学院,江苏南京,210009 |
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| 摘 要： | 目的]确定超声波法提取花生壳中白藜芦醇的最佳工艺条件。方法]以花生壳为原料,用超声波法对白藜芦醇的提取工艺进行研究,考察了提取时间、乙醇浓度、占空比和料液比对提取率的影响,通过单因素和正交实验确定了最佳提取工艺条件,并通过HPLC进行定性定量的检测。结果]4因素中料液比对白藜芦醇的提取率影响最显著,其次为乙醇体积分数和提取时间,占空比的影响最小。通过单因素和正交实验确定了最佳提取条件:提取剂为70%乙醇,超声7 min,占空比50%,料液比为1:15,白藜芦醇一次提取率达12.99μg/g。用HPLC法定性定量检测,结果准确且高效。结论]以乙醇为提取剂,用超声波法从花生壳中提取白藜芦醇,是一种高效环保型工艺,具有广阔的应用前景。
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| 关 键 词： | 超声波提取法 白藜芦醇 花生壳 HPLC |
Ultrasonic Extraction and HPLC Detection of Resveratrol Extracted from Peanut Shell |
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| LIU Chang-jin et al.Ultrasonic Extraction and HPLC Detection of Resveratrol Extracted from Peanut Shell[J].Journal of Anhui Agricultural Sciences,2009,37(28). |
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| Authors: | LIU Chang-jin |
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| Institution: | LIU Chang-jin et al(College of Food Engineering , Biotechnology,Tianjin University of Science , Technology,Tianjin 300457) |
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| Abstract: | Objective]The research aimed to confirm the best process conditions of resveratrol extracted from peanut shell with ultrasonic wave method.Method]With peanut shell as raw material,the extraction process of resveratrol with ultrasonic wave method was studied,the effects of extracting time,ethanol conch.,duty cycle and solid-liquid ratio on extraction rate were investigated,and the optimal extracting process conditions were confirmed through single factor and orthogonal experiment,and qualitative and quanti... |
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| Keywords: | HPLC |
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