| 宜昌百合的组织培养研究 |
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| 引用本文： | 熊丹,陈发菊,梁宏伟,王玉兵,张德春. 宜昌百合的组织培养研究[J]. 福建林业科技, 2007, 34(4): 91-94,97 |
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| 作者姓名： | 熊丹 陈发菊 梁宏伟 王玉兵 张德春 |
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| 作者单位： | 三峡大学生物技术研究中心/湖北省天然产物研究与利用重点实验室,湖北,宜昌,443002 |
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| 基金项目： | 湖北省教育厅重点项目(NO.SD200216)，宜昌市科技局重点攻关项目(NO.A06209)资助 |
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| 摘 要： | 试验以宜昌百合的鳞茎为外植体,研究宜昌百合组织培养快繁的最佳方法。结果表明:宜昌百合鳞茎的最适诱导培养基为:MS+6-BA 1.0 mg.L-1+NAA 2.0 mg.L-1,添加20%的椰乳和0.5%的活性炭,同时可以有效地防止宜昌百合在培养时产生褐化;较适的生根培养基为:1/2MS+0.6 mg.L-1IBA+0.5%AC。该实验结果为宜昌百合的快速繁殖及工业化生产提供了理论基础和技术支持。
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| 关 键 词： | 宜昌百合 组织培养 激素 活性炭 椰乳 |
| 文章编号： | 1002-7351（2007）04-0091-04 |
| 收稿时间： | 2007-04-10 |
| 修稿时间： | 2007-04-10 |
Studies on the tissue culture of Lilium leucanthum |
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| XIONG Dan,CHEN Fa-ju,LIANG Hong-wei,WANG Yu-bing,ZHANG De-chun. Studies on the tissue culture of Lilium leucanthum[J]. Journal of Fujian Forestry Science and Technology, 2007, 34(4): 91-94,97 |
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| Authors: | XIONG Dan CHEN Fa-ju LIANG Hong-wei WANG Yu-bing ZHANG De-chun |
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| Abstract: | To develop a suitable approach for the rapid propagation of Lilium leucanthum by tissue culture,bulbs were tried as the explant.The result showed that the suitable medium for the bulb explant culture was MS+6-BA 1.0 mg·L-1+NAA 2.0 mg·L-1,supplied with 20% coconut milk and 0.5% activated charcoal(AC) which prevented from browning;The suitable rooting medium was 1/2MS+0.6 mg·L-1 IBA +0.5% AC.Tissue culture of Lilium can achieve its rapid propagation,resulting in the possibility for its industrial production. |
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| Keywords: | Lilium leucanthum tissue culture hormone activated carbon coconut milk |
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