小叶朴组织培养无菌体系的建立

目的]建立小叶朴组织培养初代无菌体系。方法]通过对不同消毒剂及消毒时间的对比,筛选出能够有效控制小叶朴初代培养的污染率,且不抑制小叶朴茎段萌发的消毒处理。结果]小叶朴茎段经70%乙醇消毒30 s 2次,再用氯化汞消毒6 min所得茎段污染率低,萌发率高。对于后期出现的真菌污染采用多菌灵作为消毒剂和添加剂,用200 mg/L多菌灵浸泡外植体60 min能有效地控制污染,且具有较高的萌发率。在培养基中添加200 mg/L多菌灵也能抑制真菌污染,且效果优于多菌灵浸泡处理。结论]建立了小叶朴组织培养无菌体系,为小叶朴组培苗的增殖和生根提供参考。

The Establishment of Tissue Culture Sterile System of Celtis bungenana

Objective] The aim was to establish tissue culture sterile system of Celtis bungenana.Method]Through the comparison of differ-ent disinfectants and disinfection time, tissue culture sterile system of Celtis bungenana with lowerpollution rate and higher germination rate were screened. Result] By comparison with 70% alcohol disinfection 30 s 2 times, with mercuric chloride for 6 minutes from the stem seg-ments were of low pollution, high germination rate.Late for the fungus pollution using carbendazim as disinfectant and additives, soaking with 200 mg/L carbendazim explant 60 min, can effectively control the pollution, and can have better germination rate.200 mg/L is added in the culture medium carbendazim can restrain fungus contamination, and better than carbendazol soaking process. Conclusion] The study stabli-hed tissue culture sterile system of Celtis bungenana,and provide reference for proliferation and rooting of Cetlis bungenana.