何鲁牵牛的组培快繁研究 |
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引用本文: | 吕永平,王燕,牟豪杰,汪一婷,李海营. 何鲁牵牛的组培快繁研究[J]. 安徽农业科学, 2016, 0(30). DOI: 10.3969/j.issn.0517-6611.2016.30.039 |
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作者姓名: | 吕永平 王燕 牟豪杰 汪一婷 李海营 |
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作者单位: | 浙江省农业科学院病毒学与生物技术研究所,浙江杭州,310021 |
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基金项目: | 国际先进农业科技术计划(948)项目(2011-G31);浙江省农业科学院青年人才培养项目(2015R21R08E08)。 |
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摘 要: | [目的]建立何鲁牵牛的组培快繁体系,为其规模化生产提供理论依据。[方法]以何鲁牵牛茎段为外植体,研究何鲁牵牛不定芽增殖和生根的最适培养基。[结果]何鲁牵牛在WPM+0.3 mg/L 6-BA+0.01 mg/L NAA+0.1 g/L活性炭的启动培养基上成功诱导腋芽抽出,将腋芽转接到WPM+0.3 mg/L 6-BA+0.1 g/L活性炭培养基上进行不定芽的增殖,增殖率达4.30;在WPM+0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L活性炭培养基上生根率较高,可达90%。[结论]该研究建立了何鲁牵牛的组培快繁体系,有利于何鲁牵牛种质资源保护和工厂化生产。
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关 键 词: | 何鲁牵牛 组培快繁 增殖 生根 |
Study on Tissue Culture and Rapid Propagation of Ipomoea holubii |
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Abstract: | Objective] To establish tissue rapid propagation system of Ipomoea holubii,and to provide theoretical foundation for its large-scale production.[Method] Taking the stem of Ipomoea holubii as explants,we researched the optimal culture medium for adventitious buds prolifera-tion and rooting of I.holubii.[Result] Axillary buds were induced on WPM+0.3 mg/L 6-BA +0.01 mg/L NAA +0.1 g/L active carbon (AC).The shoots of I.holubii were cultured on WPM +0.3 mg/L 6-BA +1 g/L AC for multiplication,and the multiplication ratio was 4.30. WPM +0.1 mg/L IBA+0.1 mg/L NAA+0.1 g/L AC was the optimal medium for rooting,and the rooting rate was 90%.[Conclusion] This re-search establishes the tissue culture and rapid propagation system of I.holubii,which is helpful to the germplasm protection and industrial produc-tion of I.holubii. |
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Keywords: | Ipomoea holubii Rapid propagation Multiplication Rooting |
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