响应面法优化酶解海洋低值鱼肉制备抗氧化肽工艺

目的]优化碱性蛋白酶制备海洋低值鱼抗氧化肽的工艺。方法]以DPPH自由基清除率为指标,在酶解温度、pH、加酶量、底物浓度等条件下进行单因素试验,在此基础上运用响应面法优化碱性蛋白酶酶解低值鱼肉制备抗氧化肽的工艺条件。结果]在温度54℃、pH 8.8、底物浓度200 g/L、加酶量2 500 U/g的条件下酶解3 h,得到抗氧化肽的DPPH自由基清除率理论值为62.66%,实际值为61.87%,相对误差为1.26%。结论]该研究为低值鱼抗氧化肽的开发利用提供理论依据。

Optimization of Enzymatic Hydrolysis of Antioxidant Peptides from Low Value Fish by Response Surface Methodology

Objective] Optimization of antioxidant peptides from marine low value fish by alkaline protease was studied.Method] The enzy-matic hydrolysis conditions for the preparation of antioxidant peptides were optimized using the response surface methodology based on single factor experiments, including hydrolysis temperature, hydrolysis pH, enzyme dose and substrate concentration. Result] The results showed that the optimal enzymatic hydrolysis conditions were as follows:substrate concentration 200 g/L, enzyme dose 2 500 U/g, pH 8.8, hydroly-sis temperature 54 ℃for 3 h, under these conditions the theoretical value of DPPH free radical scavenging rate was 62.66%, the actual value was 61.87%, the relative error was 1.26%.Conclusion] The study can provide theoretical basis for development and utilization of antioxi-dant peptide of low value fish.