水产品中氟喹诺酮类药物残留的测定

目的]建立水产品中诺氟沙星、环丙沙星、恩诺沙星3种氟喹诺酮类药物残留测定的超高效液相色谱法。方法]以0.10%甲酸-乙腈为提取溶剂,提取液经浓缩,正己烷净化去脂,采用超高效液相色谱-荧光检测器检测3种氟喹诺酮类药物,外标法定量。结果]该方法的相对标准偏差为1.4%~8.1%,回收率为82%~106%,3种氟喹诺酮类药物在0.01~1.00μg/m L的浓度范围内具有良好的线性关系,相关系数均大于0.995,方法检出限为5.00μg/kg。结论]该检测方法准确、快捷、高效,可广泛应用于水产品中氟喹诺酮类药物残留的检测。

Determination of Quinolones Residues in Aquatic Products

Objective]An ultra performance liquid chromatography method was developed for the simultaneous determination of 3 quinolone antibiotics in norfloxacin, ciproxacin, enrofloxacin.Method] The method with 0.10% formic acid and acetonitrile as extraction solvent, the extract was concentrated and degreased by hexane, 3 kinds of quinolones antibiotics were detected by ultra-liquid chromatographic with fluores-cence detector, and were quantified using external standard method.Result] The relative standard deviations(RSD) were 1.4%-8.1%;recoveries were 82%-106%;a variety of quinolone antibiotics showed good linearity in the concentration range of 0.01-1.00 μg/mL, cor-relation coefficients were greater than 0.995;detection limits were 5.00 μg/kg.Conclusion] The detection method is accurate, fast and effi-cient, and can be widely used in the daily detection of quinolone antibiotics in aquatic products.