脱毒菜籽多肽对小鼠免疫功能的影响

[目的]研究脱毒菜籽多肽对小鼠免疫功能的影响。[方法]试验组以低、中、高剂量组0.334、0.667和2.000 g/(kg·d)的脱毒菜籽多肽灌胃小鼠,对照组以等体积纯净水灌胃,30 d后进行二硝基氟苯诱导小鼠迟发性变态反应试验、Con A诱导小鼠脾淋巴细胞转化试验、抗体生成细胞检测和血清溶血素测定试验、小鼠碳廓清试验、小鼠腹腔巨噬细胞吞噬鸡红细胞试验、NK细胞活性测定试验、试验前后小鼠体重以及脏器/体重比值试验。[结果]脱毒菜籽多肽对小鼠的耳廓肿胀、脾淋巴细胞转化、吞噬指数a和NK细胞活性均有显著影响,但对小鼠体重增长、胸腺/体重比值、脾脏/体重比值、脾抗体生成细胞量、血清溶血素水平和腹腔巨噬细胞吞噬能力均无影响。[结论]脱毒菜籽多肽具有增强免疫力的功能。

Effect of Detoxified Rapeseed Peptides on Mouse Immune Function

Objective] The research aimed to study the effect of detoxified rapeseed peptides on immune function .[ Method] Low-, medium-and high-dosage group were fed with 0.334, 0.667 and 2.000 g/( kg? d) detoxified rapeseed peptides, respectively, equal volume of pure water as control group.30 days later, the following experiments were conducted: DNFB induced mouse delayed type hypersensitivity,mouse spleen lymphocyte transformation capacity induced by ConA,antibody forming spleen cells,serum hemolysin,mouse carbon clearance ability, mouse peritoneal macrophage phagocytosis of chicken erythrocytes,NK cell activity,initial and terminal body weight of mice,viscera/body weight ratio.[Result]The detoxified rapeseed peptides on mouse carbon clearance ability, spleen lymphocyte transformation capacity, phago-cytic index a and NK cell activity had significant impact.However rapeseed peptides had no significant effect on mouse weight gain, the thy-mus/body weight ratio, spleen/body weight ratio,cells production of spleen antibody, serum hemolysin leveland function of peritoneal macro-phage phagocytosis of chicken erythrocytes.[Conclusion]The detoxified rapeseed peptides have effect on enhancing immunity.