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台蘑小香蕈液体发酵培养基筛选
引用本文:尤利霞.台蘑小香蕈液体发酵培养基筛选[J].安徽农业科学,2016,44(26):89-90.
作者姓名:尤利霞
作者单位:山西省医药与生命科学研究院,山西太原,030006
基金项目:科技部科技援外项目(KY20110097)。
摘    要:目的]优化台蘑小香蕈液体发酵培养基的最佳配方。方法]通过单因素试验考察葡萄糖、蔗糖、马铃薯汁、无机盐等常用碳源与氮源对台蘑液体发酵菌丝生物量的影响。结果]台蘑小香蕈液体发酵培养基的最佳配方为蔗糖3.0%、麸皮汁3.0%、玉米粉5.0%、马铃薯汁30%、植被汁10%、NaNO_3 0.3%、K_2HPO_40.1%、KCl 0.1%、FeSO_4 0.001%,pH 7。结论]优化后的培养基配方能明显提高台蘑液体发酵的菌丝生物量,配方经济合理。

关 键 词:台蘑  液体培养  菌丝体  碳源  氮源  无机盐

Screening of Liquid Fermentation Mediums of Small Mushrooms from Wutai Mountains
Abstract:Objective] To optimize the best formula of liquid fermentation mediums of small mushrooms from Wutai Mountains.Method] Through a single factor experiment, the effects of commonly used carbon and nitrogen sources ( glucose, sucrose, potato juice, and inorganic salt) on the mycelial biomass of small mushrooms from Wutai Mountains were studied . Result] The best formula of the liquid fermentation medium of small mushrooms from Wutai Mountains are shown as follows: sucrose3 .0%, bran juice 3.0%, corn flour 5.0%, potato juice 30%, vegetation juice 10%, NaNO3 0.3%, K2HPO4 0.1%, KCl 0.1%, FeSO4 0.001%, and pH=7.Conclusion] The optimized formula of the liquid fermentation medium can significantly improve the mycelial biomass of small mushrooms from Wutai Mountains , so it is economi-cal and reasonable.
Keywords:Small mushrooms from Wutai Mountains  Liquid culture  Mycelium  Carbon sources  Nitrogen sources  Inorganic salts
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