大青山油松根际固氮菌的多样性研究

[目的]研究大青山油松树种根际固氮菌的多样性。[方法]对从大青山油松(Pinus tabulaeformis)根际土壤中分离的固氮菌进行16S rDNA扩增及序列测定,应用DNAMAN 6.0软件进行多序列比对,使用MEGA4软件构建系统发育树。[结果]大青山油松根际土壤中分离的46株固氮菌分属于Pseudomonas、Bacillus、Phyllobacterium、Cupriavidus、Paenibacillus、Enterobacter、Agrobacterium、Arthrobacter、Naxibacter、Burkholderia、Ensifer等11个不同的类群,其中Pseudomonas、Bacillus、Arthrobacter、Phyllobacterium为优势属种。系统分析表明,大青山油松根际土壤中固氮菌具有较丰富的多样性。[结论]该研究结果可为森林生态系统的可持续发展提供理论依据。

Diversity of Nitrogen-fixing Bacteria from Pinus tabulaeformis in Daqing Mountains

Objective] The aim was to study diversity of nitrogen -fixing bacteria isolated from Pinus tabulaeformis in Daqing Mountains. [Method] To amply and sequence 16S rDNA gene of nitrogen-fixing bacteria isolated from Pinus ta bulaeformis in Daqing Mountains,the align-ment of 16S rDNA sequences was conducted by DNAMAN 6.0,and the Neighbor-joining phylogenetic tree was constructed by MEGA 4 soft-ware.[Result]The phylogenetic analysis showed that forty -six isolates from P.tabulaeformis belonged to eleven groups,which are Pseudomonas, Bacillus, Phyllobacterium, Cupriavidus, Paenibacillus, Enterobacter, Agrobacterium, Arthrobacter, Naxibacter, Burkholderia, Ensifer, and Pseudo-monas,Bacillus,Arthrobacter,Phyllobacterium are dominant groups.The results showed that the nitrogen-fixing bacteria from P.tabulaeformis in Daqing Mountains have rich diversity.[ Conclusion] The results can provide theoretical basis for sustainable development of forest ecosystem.